大肠杆菌O157:H7 IntC300 B细胞抗原表位的综合预测  

The Prediction of B-Cell Epitopes in C-Terminal of Intimin of EHEC O157:H7

作　　者：周勇[1] 吴仲鑫[1] 彭丽娟[1] 万成松[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院微生物学系,广州510515

出　　处：《热带医学杂志》2008年第1期12-14,27,共4页Journal of Tropical Medicine

基　　金：广东省科技计划项目(No.2002B3100101)

摘　　要：目的预测肠出血型大肠杆菌O157∶H7 IntiminC端300氨基酸片段(IntC300)的B细胞抗原表位。方法采用计算机软件和网络服务器分析IntC300的亲水性、β-转角、柔韧性、表面可及性和抗原指数,判断其B细胞抗原表位。结果B细胞抗原表位可能在20-36、76-88、189-198、262-279和284-296残基或其附近。结论对EHEC O157∶H7 IntC300 B细胞抗原表位的综合预测,为研究诊断性单克隆抗体及多肽疫苗提供了理论工具。Objective To predictate epitopes in C-terminal 300aa of intimin of Enterohemorrhagic Escherichia coli O157 ： H7. Methods Protein analytical program and protein analytical tools in web sites were used to predict hydrophilicity, β-turn, flexibility, accessibility, and antigenicity of C-terminal 300aa of intimin. The potential B-cell epitopes were predicted from the results. Results It showed that B-cell epitopes were probably at or adjacent to 20-36, 76-88, 189-198,262-279 and 284-296 domains. Conclusion Our study is helpful for preparation of monoclonal antibody and investigation of synthetic peptide vaccine to EHEC O157 ： H7.

关 键 词：出血型大肠杆菌O157:H7 Intimin蛋白 抗原表位 

分 类 号：R378.21[医药卫生—病原生物学]