氟西汀对强迫游泳应激大鼠行为及血清皮质酮的影响  

Effect of fluoxetine on behavior and serum corticosterone in stressed rats

作　　者：张强 柳威[2] 陈夫银[2] 

机构地区：[1]汕头大学精神卫生中心,汕头515063 [2]汕头大学司法鉴定中心

出　　处：《中国行为医学科学》2008年第1期1-3,共3页Chinese Journal of Behavioral Medical Science

基　　金：国家自然科学基金（30371578）;广东省自然科学基金（021219）;广东省医学科研基金立项课题（B2004093）

摘　　要：目的 探讨应激导致大鼠行为和血清皮质酮浓度的改变以及氟西汀的抗抑郁机理。方法将32只大鼠随机分为对照组（n=6）、4周应激给水组（n=11）和4周应激给药组（n=15），各应激组大鼠连续给予强迫游泳实验4周，每次持续15min，每天1次。游泳后给予灌胃，4周应激给药组按4mg／kg给予氟西汀生理盐水溶液灌胃，4周应激给水组按相同比例给予生理盐水灌胃，采用Open—Field法评定行为，使用酶联免疫吸附测定法（ELISA）测定血清皮质酮水平。结果应激后对照组爬格数[（79．33±34．82）次]和4周应激给药组[（73．53±43．66）次]明显高于4周应激给水组[（40．09±28．28）次，P〈0．05]，对照组与4周应激给药组比较未见差异；对照组大鼠直立次数[（15．67±12．53）次]明显高于4周应激给水组[（4．27±4．92）次，P〈0．05]，而与4周应激给药组[（10．00±11．14）次]相比差异无显著性；对照组大鼠修饰次数[（1．17±1．33）次]明显低于4周应激给水组[（4．27±3．00）次，P〈0．05]，而与4周应激给药组[（3．40±2．47）次]相比差异无显著性。对照组大便次数[（3．09±2．21）次]和4周应激给水组[（2．83±1．47）次]明显高于4周应激给药组[（0．93±1．49）次，P〈0．05]，对照组与4周应激给水组相比差异无显著性。对照组血清皮质酮[（156．94±113．04）ng/ml]明显低于4周应激给水组[（363．11±153．58）ng／ml，P〈0．01]和4周应激给药组[（315．49±146．35）ng／ml，P〈0．05]，而4周应激给水组与4周应激给药组血清皮质酮相比差异无显著性。结论 应激可以导致大鼠主动活动行为减少和皮质酮激素水平升高；氟西汀可以有效逆转应激所致行为学改变，对应激大鼠皮质酮激素水平的影响不明显。Objective To explore the mechanism of behavior and serum corticosterone concentration changes induced by stress and the antidepressive mechanism of fluoxetine. Methods 32 rats were randomly allocated into control（ n = 6） , 4 week-stress with saline （ n = 11 ） and 4 week-stress with fluoxetine （ n = 15 ） groups. Forced Swimming Test was used to set up stress animal model, and changes of behavior and concentration of serum corticosterone were measured by open-field test and ELISA. Results The crossing number of control group （79.33 ±34.82） and 4 week-stress with fluoxetine group （73.53 ± 43.66） are both higher than 4 week-stress with saline after stress[ （40.09±28.28） , P〈 0. 05 ] , while no difference was found compared with each other; the number of standing up of control group（ 15.67 ± 12.53 ） is much higher than 4 week-stress with saline group [ （4.27±4.92）, P 〈 0. 05 ], there is no difference when compared with 4 week-stress with fluoxetine group （10. 00 ± 11.14） ; compared with 4 week-stress group（4.27 ± 3.00） , the number of ornament of control group is much lower[ （ 1.17 ± 1.33 ） , P〈 0. 05 ] , there is no difference when compared with 4 week-stress with fluoxetine group（3.40± 2.47） ; the number of stool of control group（3.09 ±2.21 ） and 4 week-stress with fluoxetine group （2.83 ±1.47 ） are both higher than 4 week-stress with saline group[ （0.93 ± 1.49） , P〈 0. 05 ] , while no difference was found compared with each other. Concentration of serum corticosterone of control group[ （ 156.94 -±113. 04）ng/ml] is much lower than 4 week-stress with saline group[ （363.11 ± 153.58） ng/ml, P〈 0. 01 ] and 4 week-stress with fluoxetine group [ （315.49 ± 146.35 ） ng/ml, P 〈 0. 05 ] , there is no difference when compared with 4 week-stress with saline group and 4 week-stress with fluoxetine group. Conclusions Stress could induce decrease of active behavior and elevation of serum corticosterone concentration, fluoxetine can r

关 键 词：应激 皮质酮 行为 氟西汀 

分 类 号：R686[医药卫生—骨科学]