川芎嗪对大鼠肾缺血-再灌注后核因子-κB表达与一氧化氮合酶活性的影响  

Impact of Tertramethylpyrazine on the Expression of Nuclear Factor-κB and the Activity of NOS after Situ Renal Ischemia-Reperfusion in Rats

作　　者：汪燕[1] 马传荣[1] 贺国芳[1] 方建国[1] 杜光[1] 姜汉英[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2008年第2期128-131,共4页Herald of Medicine

摘　　要：目的研究川芎嗪对肾脏缺血-再灌注后肾功能、核因子-κB(NF-κB)的表达及一氧化氮合酶(NOS)活性的影响。方法将30只SD大鼠随机分为假手术组、缺血-再灌注模型组和川芎嗪处理组,化学法检测血清肌酐(Cr)和尿素氮(BUN)浓度以及左肾组织中一氧化氮合酶活性,HE染色后镜下观察肾脏病理变化,免疫组化测定肾组织NF-κB的表达水平。结果川芎嗪15,30,45 mg.kg-1剂量于缺血前静脉注射均能降低再灌注时肾脏组织中NF-κB的表达,抑制诱生型NOS(iNOS)活性、降低尿素氮和肌酐水平,其中以15 mg.kg-1作用最为显著。结论川芎嗪15,30,45mg.kg-1剂量均能减轻肾缺血损伤,其机制可能与抑制NF-κB的表达、降低iNOS活性有关,且该作用与川芎嗪的剂量相关,以15 mg.kg-1剂量的效果最好。Objective To observe the impact of tertramethylpyrazine（TMP） on the expression of Nuclear factor-KB and the activity of NOS after situ renal ischemia/reperfusion（I/R） in rats. Methods 30 female SD rats were randomly divided into sham operated group, model group and TMP treated group. Plasma samples were collected for Cr and BUN test. Renal tissues were collected to detect histological changes, the expression of NF-KB and the activity of NOS. Results Compared with the model group, the serum levels of Cr and BUN were decreased in the TMP group （ 15,30,45 mg ·kg^-1 ）, among which 15 mg·kg^-1 was more effective. Meanwhile, the expression of NF-KB and the activity of iNOS in renal tissues were decreased significantly. Conclusion TMP preconditioning can protect the renal function after renal I/R, and the mechanisms of which may be associated with inhibiting the activation of NF-KB and the expression of iNOS. The protective effect is dosage related, and the 15 mg ·kg^-1 seems to be the optimization.

关 键 词：川芎嗪 缺血-再灌注损伤 核因子-KB 一氧化氮合酶 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]