投氟大鼠肾组织蛋白质组学的初步研究  

Proteomical analysis of kidney of the fluoride-treated rat

作　　者：徐辉[1] 井玲[1] 张静敏[1] 李广生[1] 

机构地区：[1]吉林大学地方病研究所病理研究室,长春130021

出　　处：《中国地方病学杂志》2008年第1期30-33,共4页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金（30271156）;吉林省科技发展计划项目（20060419-3）

摘　　要：目的探讨蛋白质组学技术在投氟所致大鼠肾组织蛋白质的整体表达变化研究中的价值，为氟中毒肾损害机制的研究提供新途径。方法采用固相pH梯度（IPG）等电聚焦双向凝胶电泳方法，对投氟（100mg／L）8周和对照大鼠肾组织的蛋白质进行分离，使用Image master 2Delite图像软件分析电泳图谱．利用基质辅助激光解吸电离-飞行时间质谱仪对两组间比较具有统计学有意义的差异蛋白点进行鉴定。结果在对照组大鼠肾组织蛋白双向电泳图谱上可观察到624个蛋白点．而投氟组肾组织双向电泳图谱上可见到776个蛋白点。与对照组比较，在投氟组大鼠肾组织表达明显改变的蛋白点经质谱鉴定出13种，主要是与细胞代谢、增殖和氧化应激相关的蛋白，其中11种蛋白表达增多，2种降低。结论本实验所采用的双向电泳和质谱鉴定方法可有效分离和分析肾整体蛋白点，并反映出投氟大鼠肾组织细胞代谢旺盛、增殖活跃和氧化应激明显的特点．表明蛋白质组学技术在氟中毒肾损害研究中具有实用价值。Objective To explore the Value of renal proteomics on studying the mechanism of renal injury induced by fluoride. Methods Wistar rats were fed with drinking water containing F- 100 mg/L of sodium fluoride （NaF） for 8 weeks. The renal proteins from rats were separated by means of two-dimensional electrophoresis（2-DE）. Image analysis was carried out using Image master 2D elite software package. The significantly differential proteins were identified by matrix assisted laser adsorption/ionization-time of flight mass spectrometry （MALDI-TOF-MS）. Results The maps of 2-DE showed that about 624 and 776 protein spots were visualized respectively in kidney of the control and fluoride-treated group. The significantly differential proteins identified by MALDI-TOF-MS in the experimental group were mainly associated with cell metabolism, proliferation and oxidative stress, among which 11 spots were up-regulated and 2 down-regulated. Conclusions Renal proteins could be well separated and analyzed using 2-DE and mass spectrometry. This technique offers a new path for analyzing renal proteins, and may provide a novel approach for the studying fluorosis mechanism.

关 键 词：氟化物中毒 肾 蛋白质组学 细胞增殖 氧化性应激 

分 类 号：R686[医药卫生—骨科学]