姜黄素通过阻断NF-κB减少IL-1β诱导的内皮脂酶表达  

Curcumin attenuates IL-1β-induced endothelial lipase expression via blocking NF-κB pathway

作　　者：许灿新[1] 王春[1] 朱炳阳[1] 罗其富[1] 孙少卫[1] 高治平[1] 李凯[1] 廖端芳[1] 

机构地区：[1]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中国药理学通报》2008年第1期75-79,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30470719);湖南省教委青年重点资助课题(No99B11);湖南省教育厅科研基金资助项目(No06C710)

摘　　要：目的观察姜黄素对培养的人血管内皮细胞内皮脂酶表达的影响,并探讨其可能的作用机制。方法不同浓度的姜黄素处理HUVEC-12细胞。RT-PCR检测内皮酯酶(endo-thelial lipase,EL)mRNA的表达;Western blot检测核因子-κB抑制因子-α(inhibitor of nuclear factor-κB-α,IκB-α)蛋白的表达;间接免疫荧光检测核因子-κB(nuclear factor-κB,NF-κB)蛋白的活化。结果IL-1β处理HUVEC-12细胞可以明显上调EL mRNA的表达,同时降低胞质蛋白IκB-α的表达水平,激活核转录因子NF-κB,增加胞核蛋白NF-κB的水平。姜黄素预处理HUVEC-12细胞可以抑制IL-1β对EL的上调作用,同时逆转IL-1β诱导的IκB-α蛋白降解和NF-κB活化。结论姜黄素可以通过阻断NF-κB活化减少IL-1β诱导的人血管内皮细胞EL的表达。Aim To investigate the effect and the possible mechanisms involved in the effect of curcumin on endothelial lipase （EL） expression in human umbilical vein endothelial cells line-12 （HUVEC-12）. Methods HUVEC-12 were pretreated with different concentri-tions of curcumin （0- 100 ted with or without IL-1β. μmol·L^ -1 ） and then treated with or without IL-1β.eNODTHELIAL LIPASE（EL） expression was detected by RT-PCR analysis.The expression of inhibitor of nuclear factor-κB -α（IκB-α） was assayed by Western blot .Nuclear factor-κb（NF-κB） was analyzed by Immunofluorescence. Results IL-1β significantly induced EL expression at mRNA level as compared with control group. The IL-1β induction of EL expression was accompanied by activation of NF-κB and reduction of IκB-α. Cureumin almost completely blocked IL-1β-induced EL expression with a marked reduction of NF-κB and significant induction of IκB-α. Conclusion Curcumin inhibited IL-1β-induced EL expression via blocking NF-κB pathway in HUVEC-12 cells.

关 键 词：内皮脂酶 姜黄素 核因子-ΚB 抑制因子-α 白介素-1Β 

分 类 号：R282.71[医药卫生—中药学] R322.12[医药卫生—中医学]