番木瓜果实扩展蛋白Cp-EXP1基因表达的荧光定量PCR分析  

Quantitative Analysis of Gene Expression of Expansin Cp-EXP1 in Papaya Fruit by Real-Time RT-PCR

作　　者：牛艳梅[1] 沈文涛[1] 卢雅薇[1] 周鹏[1] 

机构地区：[1]华南热带农业大学热带生物技术研究所/热带生物技术国家重点实验室,海南海口571101

出　　处：《生物技术通讯》2008年第1期84-86,共3页Letters in Biotechnology

摘　　要：目的:对番木瓜果实扩展蛋白Cp-EXP1的基因进行荧光定量PCR分析,以便对其功能做进一步研究。方法:以不同成熟阶段的番木瓜果实总RNA为模板,设计引物进行荧光定量PCR。结果:Cp-EXP1基因在不同成熟阶段的番木瓜果实中的表达是有差异的,其表达水平受乙烯调控。结论:Cp-EXP1基因的表达与番木瓜的成熟软化密切相关,其表达分析为研究番木瓜的生长发育及其品种改良打下了良好的基础。Objective： Cp-EXP1 is the first expansin gene cloned from papaya. Real-time PCR was carried in order to research on its function. Methods： RNA was extracted from papaya fruit in different mature period, and quantitative analysis of Cp-EXP1 gene expression was carried by using Real-time PCR method. Results： It was suggested that the different mature period of papaya fruit differed in expression of Cp-EXP1, and the expression was regulated by ethylene. Conclusion： The expression of Cp-EXP1 was related to ripening and softening of papaya fruit, the quantitative analysis of CpEXP1 gene expression in papaya fruit build a great base of its growth research.

关 键 词：番木瓜果实 扩展蛋白 Cp-EXP1 荧光定量PCR 基因表达分析 

分 类 号：Q78[生物学—分子生物学] S667.9[农业科学—果树学]