各种RNA干扰库的构建及比较  

The Construction and Comparison of Different RNA Interference Libraries

作　　者：郭强[1] 孔毅荣[1] 徐俊杰[1] 陈薇[1] 

机构地区：[1]军事医学科学院病原微生物与生物安全国家重点实验室,北京100071

出　　处：《生物技术通讯》2008年第1期108-110,共3页Letters in Biotechnology

摘　　要：不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。简要介绍上述RNA干扰库的构建方法及其特点和局限。Different RNA interference libraries has been constructed and applied in gene functional studies. Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form, chemically synthesized small interference RNA（siRNA） libraries, chemically synthesized small hairpin RNA（shRNA） libraries, enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries. In this paper a review of four construction approaches and a discussion of their strengths and limitations were given.

关 键 词：小干扰RNA 小发夹RNA RNA干扰库 筛选 

分 类 号：Q752[生物学—分子生物学]