云南火焰兰种子无菌萌发和试管育苗  

Seed Germination in vitro of Renanthera imschootiana Rolfe

作　　者：林丹妮[1] 陈之林[1] 段俊[1] 吴坤林[1] 曾宋君[1] 

机构地区：[1]中国科学院华南植物园

出　　处：《热带亚热带植物学报》2008年第1期83-88,共6页Journal of Tropical and Subtropical Botany

基　　金：市科技计划项目(2004J1-C0201和2007Z3-E0631)资助

摘　　要：对云南火焰兰进行无菌播种技术试验。结果表明,适宜萌动的培养基为1/4MS+椰子水20%+蛋白胨1gL-1+蔗糖10gL-1,播种30d时萌动率可达93.1%;适宜萌发的培养基为VW+椰子水10%,培养75d时萌发率达36.6%。盐浓度较高的MS培养基不适合种子萌发和原球茎生长,低盐的KC培养基上种子能较好地萌发和成苗;添加椰子水可明显提高种子萌动率,并有利于原球茎的生长和分化。光培养和暗培养对种子萌动的影响差异不明显,但暗培养3周会对种子后期发育不利。添加150gL-1的苹果匀浆有利于幼苗生长。试管苗移栽成活率在95%以上。Asymbictic germination of an endangered orchid, Renanthera imschootiana Rolfe was achieved through sowing the seeds after pollinating for 145 d on different mediums axenically. In 30 d after sowing, the seed germination rate reached 93.1% on the 1/4MS medium supplemented with 20% （v/v） coconut milk（CM）, 1 g L^-1 peptone and 10 g L 1 sucrose. After 75 d, on the VW medium with 10% CM, 36.6% of the seeds formed plantlets. The content and concentration of multi-salt of basic medium has a positive （or great） effect on seed germination and plantlet growing. 1 g L^-1 peptone increased seed germination rate, while 150 g L^-1 apple homogenate can enhance plantlet growth. Seed germination rate was found no significant difference when seeds were sowed in light or dark, but 3-weeks culture in dark had a negtive effect on developing seedlings after seed germination. More than 95% seedlings were survival after transplanted to the greenhouse.

关 键 词：云南火焰兰 种子 无菌萌发 试管育苗 

分 类 号：S682.31[农业科学—观赏园艺]