分泌血型特异性抗体人B淋巴细胞系的建立  

Establishment of Epstein-Barr virus-transformed B cell lines from healthy volunteers of type B

作　　者：潘秀珍[1] 岳建华[1] 李先富[1] 王长军[1] 唐家琪[1] 

机构地区：[1]南京军区疾病预防控制中心,江苏南京210002

出　　处：《医学研究生学报》2008年第1期8-10,I0001,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30471600);江苏省自然科学基金资助项目(批准号:BK2003012);全军"十一五"计划项目基金资助(批准号:06MA115)

摘　　要：目的:建立Epstein-Barr病毒(EBV)转化的分泌血型特异性抗体的永生化B淋巴细胞系。方法:采用EBV转化外周血B淋巴细胞,加入环孢素抑制T淋巴细胞,建立B血型人B淋巴细胞系(B-LCL),红细胞凝集试验检测细胞培养上清与血型红细胞结合活性。结果:共采集B血型健康人全血15份,成功转化12份,转化率为80%;获得9株分泌抗A抗体的人B淋巴细胞系。结论:建立了分泌抗A抗体B淋巴细胞系,为进一步研制人源血型特异性单克隆抗体奠定基础。Objective： To develop Epstein-Barr virus （EBV）-transformed human peripheral blood B cell lines from healthy volunteers of type B. Methods： B lymphocytes from healthy volunteers of type B ca- pable of producing anti-A antibodies were transformed by EBV, while Cyclosporine A（ CsA）, as an immunosuppressive agent that could selectively inhibit T-lymphocytes and protect B-lymphocytes from regression, was used in the experiment. Then, the supernanant of the cell culture medium was tested with red blood ceils of type A, B and O by agglutinin assay. Results： Twelve of the15 EBV-transformed B lymphocyte cell lines were established, with a success rate of 80%, while 9 human B lymphocyte lines secreted anti-A antibodies. Conclusion： Human B lymphocyte lines secreting antibodies to A antigens were successfully developed, which helps further studies of human blood specific monoclonal antibodies.

关 键 词：EPSTEIN-BARR病毒 转化 B淋巴细胞 血型 

分 类 号：R392.1[医药卫生—免疫学]