噬菌体肽库筛选转化生长因子βⅡ型受体亲和肽  

Screening affinity peptides of transforming growth factor beta type Ⅱreceptor from the phage display peptide library

作　　者：胡义[1] 王庆林[1] 

机构地区：[1]湖南师范大学医学院寄生虫病研究室,湖南长沙410006

出　　处：《医学研究生学报》2008年第1期19-21,共3页Journal of Medical Postgraduates

基　　金：湖南省教育厅科研基金资助项目(批准号:04C392)

摘　　要：目的:从噬菌体随机肽库中筛选转化生长因子β(TGF-β)Ⅱ型受体的亲和短肽。方法:以重组可溶性人TGF-βⅡ型受体作为靶标,应用噬菌体随机十二肽库进行筛选,经过3轮淘选,提取阳性噬菌体克隆ssDNA,测序并进行序列分析。结果:通过筛选噬菌体获得富集,挑选10个能与人TGF-βⅡ型受体特异性结合的噬菌体克隆,测序得到4个核酸序列,未发现共有保守序列。结论:通过噬菌体肽库技术能筛选出与TGF-βⅡ型受体结合的噬菌体展示肽,为进一步研究TGF-βⅡ型受体亲和短肽及在抗肝纤维化中的作用提供依据。Objective： To screen high-affinity short peptides of the transforming growth factor beta type Ⅱ （TGF-β Ⅱ ） receptor from the phage display 12-mer peptide library. Methods： The recombinant solube human TGF-β Ⅱ receptor was used as the target to screen its affinity short peptides from the phage display 12-mer peptide library. The positive phage clones were obtained by 3 rounds of biopanning, followed by single stranded DNA extraction and sequence analysis. Results： Four nucleotide sequences were obtained from 10 positive phage clones with high-affinity TGF-β Ⅱ receptor. Neither conservative nor homologous sequence was found by sequence analysis compared with the transforming growth factor beta 1. Conclusion： Phage short peptides have been screened from the phage display library, which helps pave the ground for further researches on affinity short peptides and their role in inhibiting hepatic fibrosis.

关 键 词：转化生长因子βⅡ型受体 亲和短肽 噬菌体随机十二肽库 肝纤维化 

分 类 号：Q782[生物学—分子生物学]