Angiopietin-2在胎儿生长受限患者胎盘组织中的表达及意义  

The expression of angiopoietin-2 and its significance in placenta of pregnant women with FGR

作　　者：李帆[1] 冯玲[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中国优生与遗传杂志》2008年第2期34-36,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的检测胎儿生长受限(FGR)患者胎盘组织中血管生成素-2(angiopoietin-2,Ang-2)的表达水平,并探讨其与FGR的关系。方法采用免疫组织化学方法(SP法)和逆转录聚合酶链反应(RT-PCR)检测FGR组和对照组的胎盘组织中Ang-2表达的定位和表达量的差异。结果1.S-P:(1)Ang-2在胎盘的绒毛滋养细胞、血管内皮细胞及血管周围细胞均有表达。(2)FGR组Ang-2的平均光密度较正常对照组显著下降(P<0.05)。2.RT-PCR:FGR组Ang-2 mRNA的表达量下降,于正常对照组相比有明显意义(P<0.05)。结论妊娠晚期Ang-2水平显著下降可能是FGR发病机制中的一个重要因素。Objective： To detect the expression of angiopoietin -2 （Ang -2） in placentas, and to evaluate the role of Ang -2 in FGR. Methods： Immunohistochemistry （SP） was used to investigate the distribution and location of angiopoietin -2 and the semi - quantitative method of reverse transcription polymerase chain reaction （ RT - PCR） was employed to investigate the mRNA expression levels of angiopoietin- 2 in placenta tissues of normal pregnant women and women with FGR. Results： 1. S- P： （1） Ang- 2 was located in trophoblast, endothelial cells and paravascular cells of placentas. （2） The mean density of angiopoietin -2 in group of FGR was significantly lower than that in normal group （ P 〈0. 05）. 2. RT - PCR： Compared with the normal group, the mRNA expression levels of angiopoietin -2 was reduced （ P 〈0.05）. Conclusion： The expression of Ang -2 was greatly reduced in late pregnancy, It maybe play an important role in the pathogenic causes of preeclampsia.

关 键 词：胎儿生长受限 血管生成素-2 SP法 逆转录聚合酶链反应 

分 类 号：R714.5[医药卫生—妇产科学]