乌头DNA的改良CTAB提取和ISSR检测  

A Modified CTAB Extraction and Inspection for Genome of Aconitum carmichael

作　　者：张岳峰[1] 万里翔[1] 徐敏[1] 侯大斌[1] 

机构地区：[1]西南科技大学生命科学与工程学院药用植物研究室,四川绵阳621010

出　　处：《湖北农业科学》2008年第1期12-14,共3页Hubei Agricultural Sciences

基　　金：四川省农作物育种攻关课题(2006YZGG12-22);绵阳市重点科技项目(06S041-05)

摘　　要：采用改良的CTAB法与常规CTAB法对23种不同来源的乌头基因组进行提取,通过紫外、电泳、PCR-ISSR等检测方法进行比较。结果表明,改良的CTAB方法得到的DNA浓度和纯度较高,无蛋白质和多糖等杂质影响,并且可以很好地应用于ISSR分子标记分析。The extracted DNA from 23 genomes of Aconitum carmnichael, by the modified CTAB method and conventional CTAB method were compared in this study. The results showed that the extracted DNA by modified CTAB method was in high purity and had no protein or amylase pollution, which could be used for ISSR molecular marker analysis.

关 键 词：乌头 CTAB DNA提取 

分 类 号：S567.239[农业科学—中草药栽培] Q503[农业科学—作物学]