冬虫夏草菌丝体液体培养基的优化  

Optimizing on liquid culture media of Cordyceps sinensis mycelia

作　　者：赵润[1] 郭成金[1] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300387

出　　处：《天津师范大学学报（自然科学版）》2008年第1期8-11,共4页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市科学技术委员会资助项目(06ZHCXNC05900)

摘　　要：采用正交分析方法对冬虫夏草菌丝体的液体培养基配方进行了优化，结果表明，冬虫夏草生长最适液体培养基配方为：葡萄糖1．25％，蔗糖1．25％，蛋白胨0．02％，酵母粉0．0625％，KH2PO4 0．025％，MgSO4·7H2O 0．0125％，VB10．0025％，pH自然；培养温度24℃，周期192h，其菌丝体生物量产率为19．5g／L，是前人菌丝体生物量产率的1．2～1．5倍．The liquid culture media of Cordyceps sinensis mycelia was optimized by means of orthogonal analysis. The results showed that the optimal medium for the mycelia was 1.25% glucose, 1.25% sucrose, 0.02% peptone, 0. 062 5% yeast powder, 0. 025% KH2PO4, 0. 012 5% MgSO4· 7H2O, 0, 002 5% VB] and pH natural. In the cultural condition of 24℃ and 192 h, the mycelia biomass reached to 19.5 g/L, which was as 1.2～1.5 times as the amount with the previous way.

关 键 词：正交分析 冬虫夏草 菌丝体 液体培养基 优化 生物量 

分 类 号：Q93-335[生物学—微生物学]