时间分辨荧光免疫技术在乙肝病毒标志物检测中的应用  

The Clinical Application of Time-resolved Fluoroimmunoassay in Detecting HBV-Markers

作　　者：朱天礼[1] 张葵[1] 

机构地区：[1]南京大学医学院附属鼓楼医院检验科,210008

出　　处：《临床输血与检验》2008年第1期23-25,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的以微粒子酶免疫分析(MEIA)为参照,探讨时间分辨荧光免疫技术(TRFIA)定量测定乙肝病毒标志物的临床应用价值。方法采用TRFIA与酶免疫吸附试验技术(ELISA)对623份随机标本做5项乙肝病毒标志物测定,其中95份标本用MEIA复测。结果TRFIA法检测乙肝五项指标与MEIA法比较,差异无显著性(P>0.05)。结论TRFIA法测定乙肝五项指标的相对特异性、灵敏度及符合率较ELISA法高,为临床疗效观察及乙肝疫苗的接种提供科学依据。Objective Based on microparticle enzyme immunoassay（MEIA） as reference method,to evaluate the clinical application of time-resolved fluoroimmunoassay （TRFIA）in the quantitation of HBV-Markers. Methods Using HBV-Markers of 623 individuals were detected by TRFIA and enzyme linked immunosorbent assay respectively. 95 cases were also detected by MEIA. Results There was no significant difference between TRFIA and MEIA methods（P〉0.05）. Conclusion The relative specificity and sensitivity of TRFIA is better than that of ELISA in HBV-Markers quantitative assay. It can provide scientific proof for evaluating the effect of treatment of type B hepatitis and inoculation of HBV vaccine.

关 键 词：微粒子酶免疫分析 时间分辨荧光免疫技术 乙肝病毒标志物 

分 类 号：R392.11[医药卫生—免疫学] R512.62[医药卫生—基础医学]