延长实验温育时间对FAME全自动酶免分析结果的影响  

Effect of the Test Results of ELISA by Prolong Incubative Time with FAME System

作　　者：帅敏[1] 张玲[1] 邓晓琴[1] 杨永红[1] 易钢[2] 向艳玲[1] 

机构地区：[1]四川省乐山市中心血站检验科,614000 [2]重庆医科大学检验系

出　　处：《临床输血与检验》2008年第1期28-30,共3页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的探讨延长温育时间对FAME全自动酶免分析系统进行酶联免疫吸附实验(ELISA)结果的影响,提高ELISA法检测结果的准确性。方法根据FAME孵育巢温度变化曲线延长ELISA实验温育时间,在不同实验参数条件下进行对照实验,根据实验结果选择合适补偿时间,所得数据采用SPSS13.0软件进行双变量相关分析。结果HBsAg阳性标本、阳性对照、0.5和1ng/ml质控对照的OD值与温育时间延长成正相关,阴性标本和阴性对照的OD值与温育时间延长的相关性无显著性。结论适当延长温育时间可提高FAME全自动酶免分析系统进行ELISA实验分析结果的准确性。Objective To study the effects of prolonging incubative time in ELISA test with FAME system and improve the accuracy of ELISA test results. Methods To observe the temperature change in incubator slot and draw the temperature change diagram,then to set different test parameters according to the temperature change for the control experiment. And to choose suitable incutative time based on the ELISA test results. All test data were analyzed with SPSS 13.0. Results The optical density value of positive samples,positive control sample,1 ng/ml and 0.5 ng/ml control sample were directed correlative to incubative time. The optical density value of negative sample and negative control sample were incorrelative to incubative time. Conclusion The accuracy of ELISA test with FAME system has been improved by proper prolonging incubative time.

关 键 词：FAME系统 ELISA 温育时间 

分 类 号：R446.61[医药卫生—诊断学]