HCV抗体检测临界值附近样本传播HCV风险的研究  

Research on the Spread Risk of HCV Antibody Samples Near the Cut off Value

作　　者：李瑞兰[1] 李忠平[1] 樊忠杰[1] 赵乐莹[1] 赵莉[1] 

机构地区：[1]天津市血液中心,300110

出　　处：《临床输血与检验》2008年第1期31-35,共5页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的用尽可能低的消耗,达到HCV感染早期诊断,尽量缩短ELISAHCV抗体检测的"窗口期",防止和减少经输血传播丙型肝炎的风险。方法采集初次检测抗-HCV(S/CO)样本测定值/临界值0.40～0.99的样本310份,采用"HCV抗体分片段酶联免疫确认试剂盒"进行补充旁证检测,对检测阳性及可疑阳性样本再进行HCV-PCR和RIBA及HCV-cAg检测。结果310份样本共检出单片段阳性样本25份,2个片段以上阳性样本3份,共计28份。HCV-PCR阳性3份,HCV-cAg阳性4份,RIBA检测3个条带阳性2份,1个条带阳性22份。28份样本中,抗-HCV分片段、PCR、HCV-cAg以及RIBA共同阳性1份,HCV-cAg与PCR共同阳性2份,HCV-cAg和RIBA共同1份。结论加强对抗-HCV检测阴性高值样本的重视,尽量缩短ELISA-HCV抗体检测"窗口期"。Objective To diagnose early infection of HCV with expense as low as possible and to shorten the ＂preseroconversion window phase （PWP）＂ of anti-HCV detection in present condition for decrease risk through blood spread. Methods 310 samples which anti-HCV ELISA S/CO values were 0. 4-0.99 were collected. The plasma of the 310 samples were separated and stored at -30℃. The plasma was tested by anti-HCV different fragment ELISA assay kit additionally. The positive and probable positive samples were tested further using HCV-PCR, RIBA and HCV-cAg respectively. Results Among 310 serum samples,25 serum samples had only one fragment positive, and 3 serum samples had more than two fragments positive. Among them, 3 samples were HCV-PCR positive, 4 samples were HCV-cAg positive,2 sam- ples had three lines positive by RIBA and 22 samples had only one line by RIBA. One sample of the above-mentioned 28 serum samples was detected positive by anti-HCV different fragment assay kit,HCV-PCR,HCV-cAg and RIBA simultane- ously. Two samples were positive in HCV-cAg and HCV-PCR assays. One sample was positive in HCV-cAg and RIBA assays. Conclusion In present condition, in order to detect HCV infection more earlier than ELISA-HCV antibody detection and to shorten the PWP, the anti-HCV critical value negative samples should be detected by anti-HCV different fragment assay kit and HCV-PCP, simultaneously. The combination detection of anti-HCV different fragment assay kit and HCV- PCR assay could take the place of the expensive import RIBA assay kit.

关 键 词：分片段 临界值 HCV 传播 窗口期 

分 类 号：R446.11[医药卫生—诊断学] R512.63[医药卫生—临床医学]