不同甘油浓度保护剂对冷冻精液复苏率的影响  

Impact of Cryoprotectants with Different Concentration of Glycerol on Motility of Post-thaw Human Sperm

作　　者：李玉山[1] 王全先[1] 高学敏[1] 胡耀龙[1] 杨险峰[1] 

机构地区：[1]郑州大学第三附属医院人类精子库,郑州市450052

出　　处：《医药论坛杂志》2007年第24期24-25,共2页Journal of Medical Forum

摘　　要：目的探讨精液冷冻保护剂中不同甘油浓度对冷冻精液复苏率的影响。方法分别用三种不同浓度甘油的保护剂对23例合格供精者的精液进行冷冻后复苏率的检测。结果10%甘油组冷冻复苏率为(64.4±4.1)%,8%甘油组冷冻复苏率为(69.7±4.6)%,与10%甘油组相比有显著差异(P<0.05),6%甘油组冷冻复苏率为(77.8±5.1)%,与8%甘油相比差异显著(P<0.05),与10%甘油组相比差异极显著(P<0.01)。结论用6%甘油的冷冻保护剂对人类精液的冻存效果更佳。Objective To compare the cryoprotectant containing different concentration of glycerol on the motility of post - thaw human sperm. Methods The motility of post - thaw human sperm of 23 samples were compared, which were used with different glycerol cryoprotectants. Results When the concentrations of glycerol in the cryoprotectant were 10% ,8% and 6% ,the motility rates of post - thaw human sperm were （64. 4 ± 4. 1 ） % , （ 69. 7 ±4. 6 ） % and （77. 8 ± 5. 1 ） % respectively. The motility rate of post - thaw human sperm of the group with 6% glycerol is higher significantly than the group with 8% glycerol （ P 〈0.05） ,and higher than the group with 10% glycerol great significantly （ P 〈0. 01 ）. Conclusion The best concentration of glycerol is 6% when they were used as a kind of sperm cryoprotetant.

关 键 词：甘油 冷冻保护剂 精液 复苏率 

分 类 号：R454.5[医药卫生—治疗学]