结缕草属植物种间关系的等位酶研究  

Isozyme Study on Interspecies Genetic Relationship of Zoysia Willd.

作　　者：周志芳[1] 宣继萍[2] 刘建秀[2] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省中科院植物研究所,南京210014

出　　处：《草地学报》2007年第6期577-581,共5页Acta Agrestia Sinica

基　　金：国家自然基金项目(30371011)资助

摘　　要：分析结缕草属(Zoysia Willd.)9种1变种共59份种源的苹果酸脱氢酶(MDH),酯酶(EST)及过氧化物酶(POD)三种等位酶特征,研究其种间亲缘关系,结果表明:①种内遗传分化大小依次为:沟叶结缕草(Z.matrella(L.)Merr.)>结缕草(Z.japonica Steud.)>中华结缕草(Z.sinica Hance)>朝鲜结缕草(Z.koreana Mez);②聚类结果显示:结缕草、中华结缕草、长花结缕草(Z.sinicavar.nipponica Ohwi)、大穗结缕草(Z.macrostachya Franch.)聚为一类;沟叶结缕草、细叶结缕草(Z.tenuifolia Willd.ex Trin.)先聚为一类,然后小结缕草(Z.minima Zotov)、太平洋结缕草(Z.pacifica Hotta ex Kuroki)、大花结缕草(Z.macrantha Desvaux)也聚到这一类;而朝鲜结缕草自成一类,聚类结果与外部性状特征和地理分布位置相统一;③结缕草属植物为开放式的外繁育系统,种内各种源间遗传变异大于种间变异。The characteristics of three isozyme systems （MDH,EST,POD） were investigated for 59 accessions of Zoysia Willd. belonging to 9 species and 1 variety to study the genetic relationship of them. The results show as follows： the genetic diversity was ranked as： Z. rnatrella （L.） Merr. 〉Z. japonica Steud. 〉Z. sinica Hance〉Z. koreana Mez; Z. japonica, Z. sinica, Z. sinica var. nipponia Ohwi, and Z. rnacrostachya Franch. were clustered together; Z. rnatrella, Z. tenuifolia Willd. ex Trin. were clustered to a group, then Z. minima Zotov, Z. pacifica Hotta ex Kuroki, and Z. rnacrantha Desvaux were also clustered to this group; Z. koreana was clustered to be a group by itself, the clustering result was consistent with the morphological characters and the geography distribution; Zoysia Willd. possesses the open breeding system, and the intraspecies genetic diversity was higher than interspecies.

关 键 词：结缕草属 种间关系 等位酶 

分 类 号：S812[农业科学—草业科学]