环氧氯丙烷处理对瓣膜组织结构及表面性状的影响  

Study on the structure and superficial characters of bioprosthetic valve treated with epichlorohydrin

作　　者：魏旭峰[1] 顾春虎[1] 段维勋[1] 邬晓臣[1] 赵亮[1] 宿学家[1] 董小超[1] 易定华[1] 

机构地区：[1]第四军医大学西京医院心血管外科,西安710032

出　　处：《中华实验外科杂志》2007年第12期1554-1556,1638,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金(30471716;30672086;3060013);国家"863"计划资助项目(2006AA02A138)

摘　　要：目的通过研究环氧氯丙烷(ECH)处理对瓣膜组织结构及表面性状的影响,探究ECH 处理生物瓣膜后防钙化的机制。方法通过测定 ECH 处理瓣膜组织的抗酶消化能力、茚三酮值、热皱缩温度,以及血浆蛋白吸附实验、血小板黏附实验评价 ECH 处理瓣膜组织的表面性状。结果经 ECH 处理后,抗酶消化能力增加,茚三酮值下降,热皱缩温度升高(P<0.01),且对血浆蛋白和血小板的黏附减少,表面性状改善。结论 ECH 处理能够增加瓣膜组织的交联,且改善瓣膜的表面性状,是 ECH 处理瓣膜组织防钙化、防衰坏的机制之一。Objective To define the structure and the surface characteristics of bioprosthetic valve treated with epoxy chloropropan （ECH） and to clarify the mechanism of anticalcification by ECH treatment. Methods The structure and surface characteristics of bioprosthetic valve treated with ECH were evaluated by measuring shrinkage temperature, digestion resistance to collagenase, plasma protein adsorption and platelet adhesion in vitro. The cross-linking values were determined by ninhydrin reaction. Fresh porcine valve and porcine valve treated with glutaraldehyde were tested as controls. Results Bioprosthetic valve treated with ECH showed lower plasma protein adsorption and platelet adhesion in vitro than controls. The ability of valves to resist collagnase degradation was significantly enhanced by ECH treatment. The remarkably increased shrinkage temperature and the cross-linking value were observed in ECH treated valves （ P 〈 0.01 ）. Conclusion Bioprosthetic valves treated with ECH have better surface characteristics and blood compatibility, which may be useful for mitigating calcification.

关 键 词：生物瓣膜 钙化 环氧氯丙烷 

分 类 号：R654.2[医药卫生—外科学] R197.32[医药卫生—临床医学]