不同无机碳源对粉核油球藻生长的影响  

Effects of inorganic carbon sources on growth of Pinguiococcus pyrenoidosus CCMP 2078

作　　者：刘然[1] 刘晓娟[1] 王铭[1] 徐宁[1] 李爱芬[1] 

机构地区：[1]暨南大学水生生物研究所

出　　处：《生态科学》2007年第3期227-231,共5页Ecological Science

基　　金：广东省科技攻关项目(2005B33201001)

摘　　要：研究了2种无机碳源对粉核油球藻(Pinguiococcus pyrenoidosus CCMP2078)生长的影响。结果表明,适量添加NaHCO3和通CO2对P.pyrenoidosus CCMP2078的生长均有促进作用,NaHCO3的添加方式对该藻的生长具有显著差异。一次性添加和分批添加NaHCO3的优化浓度分别为5mmoL·L-1和20mmoL·L-1,后者对数生长末期的细胞密度、干重和比生长速率均最高,分别为8.93×106cells·mL-1,0.247g·L-1和0.225d-1。通入CO2的体积分数为0.5%时,最有利于藻细胞的生长,对数生长末期的细胞密度、干重和比生长率分别为3.83×107cellsmL-1、0.42g·L-1和0.212d-1。Effects of two types of inorganic carbon sources on the growth of Pinguiococcus pyrenoidosus CCMP 2078 were investigated in this study. The results showed that the growth of P.pyrenoidosus CCMP 2078 was significantly promoted by adding appropriate amount of NaHCO3 or CO2, and there were significant differences among different additions of NaHCO3. The optimal concentrations of NaHCO3 added for one time and added equally for several times were 5 mmoL·L^-1 and 20 mmoL·L^-1, respectively. For the latter treatment, parameters of cell density, dry weight, and the specific growth rate reached to the highest value, which were 8.93×10^6 cells·mL^-1, 0.247 g·L^-1, and 0.225 d^-1, respectively. When CO2 content was 0.5%, P. pyrenoidosus CCMP 2078 reached to the highest growth rate, and parameters of cell density, dry weight, and the specific growth rate were 3.83×10^7 cells·mL^-1, 0.42 g·L-1, and 0.212 d^-1, respectively.

关 键 词：粉核油球藻 无机碳源 生长 

分 类 号：Q949.2[生物学—植物学]