彗星电泳判断川陈皮素诱导的A549细胞死亡方式  

The comet assay to determine the mode of cell death of A549 cells induced by nobiletin

作　　者：罗刚[1] 周黎明[1] 曾云[1] 杨文吉[1] 

机构地区：[1]四川大学华西医学中心药理学教研室,成都610041

出　　处：《中药药理与临床》2007年第6期10-12,共3页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:探讨川陈皮素诱导的A549细胞死亡方式。方法:40、80μg/ml的川陈皮素分别处理A549细胞12、24、46h后,用彗星电泳法来检测DNA的损伤情况,并判断细胞的死亡方式。结果:40μg/ml的川陈皮素能诱导A549细胞凋亡,而80μg/ml的川陈皮素则诱导A549细胞坏死。结论:当川陈皮素作用剂量较小时,以诱导A549细胞凋亡为主;而当作用剂量较大时,则以诱导坏死为主。To investigate the mode of cell death of A549 ceils induced by nobiletin. Methods： Comet assay was applied to determine the mode of ceil death of A549 ceils which was treated with 40μg/ml and 80μg/ml nobiletin for 12, 24 and 48h, respectively. Results： 40μg/ml nobiletin induced apoptosis of A549 ceils; however, 80μg/ml nobiletin induced necrosis. Conclusion： Nobiletin induced apoptosis at lower dose, whereas induced necrosis at higher dose.

关 键 词：川陈皮素 A549 彗星电泳 

分 类 号：R285[医药卫生—中药学]