黄芩苷对T淋巴细胞增殖与活化的影响  

The effect of baicalin on the proliferation and activation of the mouse T lymphocytes

作　　者：曾光[1] 梁清华[1] 游万辉[1] 吴汉军[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,长沙410008

出　　处：《中药药理与临床》2007年第6期20-22,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金资助项目(30371833);湖南省科学技术厅科技计划(2007SK3030)

摘　　要：目的：研究黄芩苷对小鼠T细胞体外活化和增殖的影响，并探讨其免疫调节机制。方法：以刀豆蛋白A（ConA）或佛波醇酯（PMA）＋离子霉素（ionomycin，Ion）诱导小鼠淋巴细胞中T细胞增殖，用MTr法测定细胞增殖；利用ConA或PMA诱导T细胞激活，流式细胞术检测T细胞早期活化标志CD69分子与CD25分子表达情况。结果：在ConA或PMA＋Ion刺激48h后，小鼠淋巴细胞MTT吸光度显著高于对照组（P〈0．01）。黄芩苷（10—40uM）显著降低了ConA或PMA＋Ion引起的MTT吸光度增加（P〈0．01）。在ConA或PMA刺激18h后，小鼠T细胞CD69表达率显著提高（P〈0．01），ConA或PMA刺激36小时后，T细胞CD25表达率显著提高（P〈0．01），黄芩苷（10—40uM）能显著降低活化（P〈0．01）。结论：黄芩苷可以显著抑制多克隆刺激剂ConA或佛波醇酯（PMA）介导的小鼠T细胞的活化和增殖。Aim ：To investigate the effects of baicalin on the expression of CD69, CD25, and proliferation of T cells by polyclonal stimulas in vitro, and explore the molecular mechanism of Baicalin. Methods： Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the express of CD69 and CD25 by activated T cells in vitro in response to oncanavalin （ Con A） or （PMA）. Mouse T lymphocyte＇s activation and proliferation was stimulated by ConA or PMA plus Ion, MTT assay was adopted to analyze the proliferation of mouse lymphocytes.Results： under ConA or PMA plus Ion treatment , MTT light absorption of ConA group or PMA plus Ion group was significantly higher than control group（ P 〈 0.01 ） . Baicalin could significantly reduce the value （ P 〈 0.01 ）. Baicalin inhibited the expression of CD69 and CD25 in activated T cells in response to Con A or PMA in a concentration-dependent manner. Conclution： Baicalin can significantly inhibit ConA or PMA mediated mouse T lymphocyte＇s activation and proliferation.

关 键 词：黄芩苷 T淋巴细胞CD25 CD69 流式细胞术 

分 类 号：R285[医药卫生—中药学]