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作 者:曾光[1] 梁清华[1] 游万辉[1] 吴汉军[1]
机构地区:[1]中南大学湘雅医院中西医结合研究所,长沙410008
出 处:《中药药理与临床》2007年第6期20-22,共3页Pharmacology and Clinics of Chinese Materia Medica
基 金:国家自然科学基金资助项目(30371833);湖南省科学技术厅科技计划(2007SK3030)
摘 要:目的:研究黄芩苷对小鼠T细胞体外活化和增殖的影响,并探讨其免疫调节机制。方法:以刀豆蛋白A(ConA)或佛波醇酯(PMA)+离子霉素(ionomycin,Ion)诱导小鼠淋巴细胞中T细胞增殖,用MTr法测定细胞增殖;利用ConA或PMA诱导T细胞激活,流式细胞术检测T细胞早期活化标志CD69分子与CD25分子表达情况。结果:在ConA或PMA+Ion刺激48h后,小鼠淋巴细胞MTT吸光度显著高于对照组(P〈0.01)。黄芩苷(10—40uM)显著降低了ConA或PMA+Ion引起的MTT吸光度增加(P〈0.01)。在ConA或PMA刺激18h后,小鼠T细胞CD69表达率显著提高(P〈0.01),ConA或PMA刺激36小时后,T细胞CD25表达率显著提高(P〈0.01),黄芩苷(10—40uM)能显著降低活化(P〈0.01)。结论:黄芩苷可以显著抑制多克隆刺激剂ConA或佛波醇酯(PMA)介导的小鼠T细胞的活化和增殖。Aim :To investigate the effects of baicalin on the expression of CD69, CD25, and proliferation of T cells by polyclonal stimulas in vitro, and explore the molecular mechanism of Baicalin. Methods: Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the express of CD69 and CD25 by activated T cells in vitro in response to oncanavalin ( Con A) or (PMA). Mouse T lymphocyte's activation and proliferation was stimulated by ConA or PMA plus Ion, MTT assay was adopted to analyze the proliferation of mouse lymphocytes.Results: under ConA or PMA plus Ion treatment , MTT light absorption of ConA group or PMA plus Ion group was significantly higher than control group( P 〈 0.01 ) . Baicalin could significantly reduce the value ( P 〈 0.01 ). Baicalin inhibited the expression of CD69 and CD25 in activated T cells in response to Con A or PMA in a concentration-dependent manner. Conclution: Baicalin can significantly inhibit ConA or PMA mediated mouse T lymphocyte's activation and proliferation.
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