黄芪对内皮祖细胞数量与功能及其iNOS的影响  

Effects of Astragalus on number,activity and iNOS of endothelial progenitor cells

作　　者：陈图刚[1] 谢旭东[2] 陈红娟[2] 余敏[2] 陈君柱[2] 

机构地区：[1]南昌大学第四附属医院心内科,南昌330003 [2]浙江大学医学院附属第一医院心内科,杭州310003

出　　处：《中药药理与临床》2007年第6期47-50,共4页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:观察黄芪对外周血内皮祖细胞(endothelial progenitor cells,EPCs)数量、功能及诱导型一氧化氮合酶(iNOS)的影响。方法:采用密度梯度离心法从人外周血获得单个核细胞,将其接种在人纤维连接蛋白包被的培养板上,培养7d后收集贴壁细胞,加入不同浓度黄芪注射液(10、20、50、100g/L)培养24h,同时对照组与黄芪50g/L组分别培养6、12、24、48h。激光共聚焦显微镜鉴定FITC-UEA-I和Dil-acLDL双染色阳性细胞为正在分化的EPCs,将其在倒置荧光显微镜下计数。然后分别采用MTT比色法、改良的Boyden小室、黏附能力测定实验和体外血管生成试剂盒来观察EPCs的增殖能力、迁移能力、黏附能力和体外血管形成能力,免疫印迹杂交法半定量测定iNOS含量。结果:黄芪增加外周血EPCs数量、增殖能力、迁移能力、黏附能力和体外血管形成能力,并且其数量、增殖能力、迁移能力、黏附能力和体外血管形成能力随黄芪浓度和作用时间的增加而增加,黄芪还增加EPCs中iNOS含量。结论:黄芪增加EPCs的数量、增殖能力、迁移能力、黏附能力和体外血管形成能力,与其增加iNOS含量有关。Objective ： To investigate whether astragalus has influences on number, activity, and inducible nitric oxide synthase（iNOS） of endothelial progenitor cells （EPCs）. Methods： Total mononuclear cells （MNCs） were isolated from peripheral blood by Ficoll density gradient centrifugation, and then the cells were plated on fibronectin - coated culture dishs. After 7 days cultured, attached cells were stimulated with astragalus （to make a series of final concentrations： 10,20,50,100 g/L）for 24 hours, the control group and group astragalus 50g/L cultured for 6,12,24,48h. EPCs were characterized as adherent cells double positive for DiLDL -uptake and lectin binding by direct fluorescent staining. EPCs proliferation, migration were assayed with MTT assay and modified Boyden chamber assay, respectively. EPCs adhesion assay was performed by replating those on fibronectin - coated dishes, and then adherent cells were counted. In vitro vasculogenesis activity was assayed by in vitro vasculogenesis kit. iNOS was assayed by western blot. Results ： Incubation of isolated human MNCs with astragalus increased the number of EPCs, their proliferative, migratory, adhesive and in vitro vasculogenesis capacity in a concentration and time dependent manner. Astragalus also increased EPCs＇ iNOS. Conclusion ： Astragalus increases the number, proliferative, migratory, adhesive and in vitro vasculogenesis capacity of EPCs, the effects were related to the increment of iNOS by astragalus.

关 键 词：黄芪 内皮祖细胞 诱导型一氧化氮合酶 

分 类 号：R285[医药卫生—中药学]