家蚕对浓核病毒中国株(BmDNV-3)抗性及感性品系中肠的差异蛋白质分析  

Differential expression of midgut protein in the resistant and susceptible strains of silkworm to Bombyx mori densonucleosis virus (China isolate BmDNV-3)

作　　者：包方[1] 姚勤[1] 李军[1] 刘晓勇[1] 余蔚[1] 尹慧娟[1] 陈克平[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013

出　　处：《昆虫学报》2007年第12期1219-1224,共6页Acta Entomologica Sinica

基　　金：江苏省自然科学基金项目(BK2006074);国家重点基础研究发展计划"973"项目(2005CB121000)

摘　　要：【目的】通过比较家蚕Bombyx mori抗性及感性品系的中肠蛋白质表达谱,获得家蚕对家蚕浓核病毒中国株(BmDNV-3)抗性相关的蛋白。【方法】利用双向电泳(2-DE)对感性品种华八35和抗性品种秋丰接种病毒后48h的蛋白质表达谱进行比较分析,并对其中的差异蛋白进行MALDI-TOF-TOF质谱分析,通过NCBInr和MSDB数据库进行蛋白点的鉴定和功能分析。【结果】获得重复性较好的差异蛋白点16个,其中质谱鉴定出5种蛋白,它们分别是糖基转移酶(glycosyltransferase)、糖基转移酶-S(GlcAT-S)、21.5kD小热休克蛋白(21.5kDsmall heat shock protein)、V-ATP酶(vacuolar ATPsynthase)和精氨酸激酶(arginine kinase)。这5种蛋白在抗性品系秋丰中的表达量均高于感性品系华八35。【结论】糖基转移酶和糖基转移酶-S仅在抗性品系中存在,提示它们可能是与抗性有关的蛋白。此外,增强的应激反应和能量代谢也可能与家蚕对BmDNV-3的抗性产生相关。[Objective] Obtaining the proteins related with silkworm＇s resistance to Bombyx mori densonucleosis virus by comparing the 2-DE maps of proteins from midguts of the susceptible and resistant strains of silkworm. [ Methods ] Two-dimensional electrophoresis （2-DE） was used to study the protein changes of the midgut between the non-susceptible strain （Qiufeng） and the susceptible one （Huaba35） after they were inoculated for 48 hours. The changed proteins were identified by MALDI-TOF-TOF MS. The data were analyzed in NCBInr and MSDB. [ Results] 16 differential proteins were obtained, of which 5 proteins including glycosyhransferase, CleAT-S, 21.5 kD small heat shock protein, vacuolar ATP synthase and arginine kinase were identified by mass spectrometry. The expression levels of the 5 proteins were higher in Qiufeng strain than in Huaba35. [ Conclusions] Glycosyhransferase and GIcAT-S only appeared in the resistent strain, suggesting that they might be related to the resistance of silkworm to the virus. In addition, the increased stress reaction and energy metabolism may also contribute to the Qiufeng＇s resistance to BmDNV-3.

关 键 词：家蚕 浓核病毒 抗性 2-D电泳 MALDI TOF/TOF MS 

分 类 号：Q939[生物学—微生物学] S884[农业科学—特种经济动物饲养]