雌激素受体亚型在妊娠期肝内胆汁淤积症患者胎盘的表达及意义  

The expression and significance of estrogen receptor subtype on placenta of pregnant women with intrahepatic cholestasis

作　　者：罗丹[1] 刘淑云[1] 邢爱耘[1] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041

出　　处：《现代妇产科进展》2007年第12期911-913,I0011,共4页Progress in Obstetrics and Gynecology

基　　金：国家教委博士点基金资助项目(No:20020610030)

摘　　要：目的:检测雌激素受体α(estrogen receptorα,ERα)、雌激素受体β(estrogenreceptorβ,ERβ)在胎盘组织的表达,探讨ER与妊娠期肝内胆汁淤积症(intrahepatic cho-lestasis of pregnancy,ICP)的关系。方法:采用逆转录聚合酶链反应(reverse transcription-ploymerase chain reaction,RT-PCR)技术和免疫组化技术(SP法)检测胎盘ERα、ERβ表达。结果:ICP患者胎盘ERβ在蛋白水平和mRNA水平表达均明显高于正常妊娠,差异有统计学意义(P<0.05),ERα表达差异无统计学意义(P>0.05)。结论:胎盘组织ERα和ERβ的蛋白水平与mRNA水平相一致,不存在蛋白翻译障碍;ICP可能与胎盘绒毛合体滋养细胞ERβ高表达有关。Objective： To investigate the expression and the significance of ERα and ERβ in pregnant women with ICP. Method：The expressions of ERα and ERβ were investigated by reverse transcription-ploymerase chain reaction （RT-PCR） and immunhistochemistry （ SP-assay）. Results：The protein expression and the mRNA expression of ERβ on placenta villi of pregnant women with ICP were significantly higher than those in normal pregnancy （P 〈 0.05 ） ,which the protein and the mRNA expression of ERα were negative significance comparatively （P 〉 0. 05 ）. Condusion： The protein expression of ERα and ERβ on placenta are correlation with the mRNA expression of ERα and ERβ,and there is no expression block of ERα and ERβ. It shows ICP may be correlation with the higher expression of ERβ.

关 键 词：妊娠期肝内胆汁淤积症 雌激素 雌激素受体Α β 胎盘 

分 类 号：R714.255[医药卫生—妇产科学]