Macroporous Resin Adsorption for Purification of Flavonoids in Houttuynia cordata Thunb  

作　　者：张颖 李淑芬 吴希文 赵星 

机构地区：[1]Key Laboratory for Green Chemical Technology of State Education Ministry,School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China

出　　处：《Chinese Journal of Chemical Engineering》2007年第6期872-876,共5页中国化学工程学报（英文版）

摘　　要：Flavonoids are one main kind of effective components in Houttuynia cordata Thunb., which display a wide range of pharmacological activity. In this study supercritical fluid extraction （SFE） with carbon dioxide was first used as preparat ion step to remove the volatile components, which are also active components, from Hout-tuynia cordata Thunb. Then ultrasound-assisted extraction was used to obtain the crude flavonoids and the macro-porous resin adsorption technology was further employed to purify the flavonoids. Nine kinds of macroporous resins with different properties were tested through static adsorption, and one macroporous resin labeled as D101 was selected. The effect of several factors, such as the ratio of column height to diameter, initial concentration and pH, on both flavonoids yield and content were explored by dynamic adsorption to obtain reasonable conditions of adsorption and desorption. The experimental results show that the content of fiavonoids can be above 60% with fia- vonoids recovery of 93.3 % under the optimum conditions of purification. HPLC analysis of the final flavonoids product shows it contains quercitrin, hypefin, rutin and quercetin.Flavonoids 是在 Houttuynia 英国管数据 Thunb 的一个主要有点有效的部件，它显示大量药理学活动。在这研究，有二氧化碳的超级批评液体抽取(SFE ) 首先被用作移开不稳定的部件的准备步，它也是活跃部件，从 Houttuynia 英国管数据 Thunb。然后帮助超声的抽取被用来获得粗略的 flavonoids 和宏多孔的树脂吸附技术进一步被采用净化 flavonoids。九种宏有不同性质的多孔的树脂通过静态的吸附，和一个宏被测试作为 D101 标记的多孔的树脂被选择。几个因素的效果例如到直径，起始的集中和 pH 的列高度的比率，被动态吸附在 flavonoids 产量和内容上探索获得吸附和解吸附作用的合理条件。试验性的结果证明 flavonoids 的内容能与在纯化的最佳条件下面的 93.3 % 的 flavonoids 恢复超过 60% 。最后的 flavonoids 产品的 HPLC 分析证明它包含橡素，在亢奋，卢丁和橡黄素。

关 键 词：FLAVONOIDS Itouttuynia cordata Thunb. macroporous resin ADSORPTION supercritical fluid extraction 

分 类 号：O647.3[理学—物理化学]