紫外分光光度法测定珍珠透骨草中总黄酮的含量  

MeasuringTotal Flavonoids in Speranskia tuberculata Using UV

作　　者：王丹[1] 孙佳明[1] 王贵金[1] 张辉[1] 

机构地区：[1]长春中医药大学中医药与生物工程研发中心,长春130117

出　　处：《世界科学技术-中医药现代化》2007年第6期61-63,共3页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：吉林省科技厅重点项目(20060910-02);长白山区域民间药珍珠透骨草黄酮类成分抗炎;抗氧化作用的研究

摘　　要：目的:建立珍珠透骨草中总黄酮的含量测定方法。方法:采用紫外分光光度法,以芦丁作对照品,测定珍珠透骨草中总黄酮的含量,检测波长为510nm。结果:珍珠透骨草中总黄酮的平均含量为1.42%,总黄酮浓度在0.008～0.040mg/mL范围内与吸收度呈良好的线性关系(r=0.9999,n=5),平均回收率为99.86%(RSD=1.21%,n=6)。结论:该法简便、快速、稳定、可靠,可用于测定珍珠透骨草中总黄酮的含量。The study is designed to establish a method for measuring the total flavonoids in Speranskia tuberculata. In the study, the contents of total flavonoids in Speranskia tuberculata were measured using UV-spectrophotometry with a detection wavelength of 510nm, borrowing the Rutin as a contrast. Test results show that Speranskia tuberculata has a total flavonoids of 1.42%, with a fine linear relationship in the range of 0.008-0.040mg/mL （r=0.9999）, an average recovery 99.86%, and an RSD of 1.21%（n = 6）. Authors believe that the method is simple, quick, stable, and reliable, desirable for measuring the total flavonoids in Speranskia tuberculata.

关 键 词：珍珠透骨草 总黄酮 芦丁 紫外分光光度法 含量 

分 类 号：R927.2[医药卫生—药学] R284.1