大剂量5-FU致肠黏膜严重损伤时肠上皮细胞msi-1的表达及其意义  

The expression of musashi-1 in small intestinal mucosa severely damaged by high dose 5-FU

作　　者：罗育其[1] 吴承堂[1] 闻英[1] 刘晋峰[1] 

机构地区：[1]南方医科大学南方医院普通外科,广州510515

出　　处：《中华普通外科杂志》2007年第12期939-942,共4页Chinese Journal of General Surgery

基　　金：广东省自然科学基金课题(032901)

摘　　要：目的观察大剂量5-FU 致肠黏膜严重损伤时肠上皮细胞 musashi-1(msi-1)的表达及其意义。方法成年 C57BL/6J 小鼠40只,分为对照组(n=8,D 组)和处理组(n=32)。D 组给予腹腔注射 PBS,处理组给予腹腔注射大剂量5-FU(150 mg·kg^(-1)·d^(-1)×5 d),分别于处理后1 d(A组)、3 d(B 组)、5 d(C 组)剖杀濒死小鼠取出小肠,用来常规 HE 染色,免疫组化检测肠上皮干细胞标记物 msi-1的表达;制备 A 组小鼠肠黏膜细胞悬液,流式细胞术检测 msi-1阳性细胞比例。结果大剂量5-FU 作用后,小鼠肠黏膜被严重破坏,黏膜萎缩,绒毛、隐窝结构消失;免疫组化结果显示msi-1表达上升,msi-1阳性细胞比例显著增高(P<0.01);流式检测提示 A 组小鼠肠黏膜细胞悬液中前向角(FSC)数值较大的细胞群 msi-1阳性细胞比例较高,约为67.75%。结论小鼠大剂量5一Fu腹腔注射后,肠黏膜细胞中表达 msi-1的肠上皮干细胞比例大幅增加,可为肠上皮的进一步分离纯化及其生物学特征的研究奠定基础。Objective To investigate the expression of musashi-1 （msi-1） and its significances in small intestinal mucous severely damaged by high dose 5-FU. Methods Total 40 adult C57BL/6J mice were divided into control group （ n = 8, group D） and experimental group （ n = 32）. Mice in control group received intraperitoneal injection of PBS as control, and mice in experimental group were intraperitoneally injected high dose 5-FU （ 150 mg per kg of body weigh for five days）. After treatment 1 day （ group A）, 3 days （group B）and 5 days（ group C）, the dying mice were killed, HE straining and immunohistochemical technique were carried out for detecting the expression of putative marker of intestinal epithelial stem cells musashi-1 （ msi-1 ） in the samples of the meddle intestine, and the percentage of the msi-1 positive cells from the intestinal mucosal cells of the mice in group A was detected by flow cytometry. Results After the treatment of high dose 5-FU, the intestinal mucous was damaged severely; the number of msi-1 positive cells increased markedly; The intestinal mucosal cells can be divided into two groups by flow cytometry, and in the group in which the value of FSC was higher, the percentage of msi-1 positive cells increased to 67.75%. Conclusions After the treatment of high dose of 5-FU, the percentage of intestinal stem cells increased significantly; this model may be useful for further isolation and enrichment of intestinal epithelial stem cells.

关 键 词：干细胞 肠黏膜 MUSASHI-1 5-FU 

分 类 号：R656.7[医药卫生—外科学]