巨噬细胞移动抑制因子和细胞周期蛋白D1的表达与肝细胞癌生长和转移的关系  

Correlations of expressions of macrophage migration inhibitor factor and cyclin D1 with tumor size and metastasis of hepatocellular carcinoma

作　　者：夏金堂[1] 李雯[2] 刘云建[2] 陈连周[2] 伍兆峰[1] 张珑涓[2] 汪谦[2] 

机构地区：[1]广州医学院附属广州市第一人民医院肝胆外科,510180 [2]中山大学附属第一医院外科实验中心

出　　处：《中华肝脏病杂志》2007年第12期918-921,共4页Chinese Journal of Hepatology

基　　金：广东省自然科学基金（5001776）;广州市科技计划项目（200523-E0381）

摘　　要：目的研究巨噬细胞移动抑制因子（MIF）、细胞周期蛋白D1（Cyclin D1）、细胞周期蛋白依赖激酶4（CDK4）、磷酸化视网膜母细胞瘤易感基因产物蛋白（phospho—Rb）在HeC组织中的表达及其与癌细胞生长和转移的关系。方法应用组织芯片技术和免疫组织化学方法检测93份HCC组织中MIF、Cyclin D1、CDK4和phospho—Rb的表达，分析它们的表达与HCC临床病理特征的关系。结果93份HCC组织中MIF、Cyclin D1、CDK4和phospho—Rb的表达率分别为71％、41％、82％和14％，MIF和Cyclin D1阳性表达率与正常肝脏组织表达率之间的差异有统计学意义（P〈0．01），CDK4和phospho—Rb阳性表达率与正常肝脏组织表达率之间的差异无统计学意义（P〉0．05）。在≥3．5cm的肿瘤中MIF表达率为79％，明显高于在〈3．5cm肿瘤中的表达率48％（P〈0．01）；有转移的肝癌组织中Cyclin D1阳性率为62％，明显高于无转移组织中的阳性率35％，差异有统计学意义（P〈0．05）。MIF表达强弱与Cyclin D1的表达呈正相关关系（P〈0，01）。CDK4和phospho—Rb的表达与肿瘤大小及是否转移的关系无统计学意义。结论MIF和Cyclin D1在HCC发展进程中可能促进肿瘤的生长和转移。Objective To explore the possible relationship between the expressions of macrophage migration inhibitor factor （MIF）, cyclin D 1, cyclin-dependent kinase 4 （CDK4）, phosphorylated-retinoblastoma susceptibility gene product Rb protein （phospho-Rb） and the development of hepatocellular carcinoma （HCC）. Methods 93 HCC tissues and 5 normal liver tissues were used to investigate the expressions of MIF, cyclin D1, CDK4 and phospho-Rb by tissue microarray and immunohistochemistry methods. Results The expression rates of MIF, cyclin D1, CDK4 and phospho-Rb in the HCC tissues were 71%, 41%, 82% and 14% respectively, and in the normal liver tissues, they were 0%, 0%, 80% and 20% respectively. The expression rates of MIF and cyclin D1 were significantly different between the tumor and the normal liver tissues and the expression rates of CDK4 and phospho-Rb were not significantly different between the tumor and the normal liver tissues. The rate difference （69% versus 48%） of MIF expression between the larger tumors （〉 3.5 cm） and the smaller tumors （〈 3.5 cm） was of statisteal significance （P 〈 0.01）. The expression rate （62%） of cyclin DI in the tumors with metastasis was significantly higher than the expression rate （35%） in the tumors without metastasis （P 〈 0.05）. MIF expression was positively correlated with cyclin D1 expression in the tumor tissues （P 〈 0.01）. CDK4 and phospho-Rb expressions were not significantly associated with the tumor sizes and metastasis status. Conclusion Our results indicate that MIF and cyclin D1 might be related to the growth and metastasis of HCC.

关 键 词：癌 肝细胞 巨噬细胞游走抑制因子 细胞周期蛋白D1 免疫组织化学 

分 类 号：R686[医药卫生—骨科学]