广西猪人肉孢子虫实验感染研究  

Experimental Infection of Sarcocystis suihominis in Pig and Human Volunteer in Guangxi

作　　者：李锦辉[1] 林珍[1] 杜进发[1] 覃业新[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第6期466-468,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：广西壮族自治区卫生厅资助项目(No.9600)~~

摘　　要：目的研究确定广西是否存在人-猪相互感染的肉孢子虫病。方法采用人-猪-人循环感染方法。取自然感染病例的粪便,用薛氏糖漂浮法收集肉孢子虫孢子囊(10000个以上),拌入饲料,用吞食法感染猪。取含成熟肉孢子虫包囊的新鲜生猪肉,剁碎,用吞食法感染志愿者(含包囊数约71000个以上)。观察感染后症状及肉孢子虫在宿主体内发育情况。结果人感染后约5h出现腹胀,8～36h腹泻水样便13次,呕吐4次,畏寒、发热(体温达38.5℃)、头昏、头痛、关节及肌肉酸痛、腰痛、上腹部隐痛,腿部抽筋,全身乏力,食欲明显减退。感染后第10天粪检查见未孢子化的孢子囊,第12天检出孢子化的孢子囊,孢子囊平均大小为11.9(8.8～14.5)μm×9.2(7.5～12.5)μm。猪感染后第5～8天轻度厌食,疲乏无力、便秘、毛疏松,第17天恢复正常。包囊平均大小为299.2(175～575)μm×62.3(30～102.5)μm。缓殖子大小为11.5(9.5～13.5)μm×4.1(2.8～5.0)μm。人感染后第46天口服乙酰螺旋霉素(0.2g/次,4次/d,连服15d),服药后第30天粪检孢子囊阴转。结论广西存在猪-人相互感染的肉孢子虫病。Objective To confirm existence of Sarcocystis suihominis and possible transmission cycle between human and pigs. Methods Based on the human-pig-human infection cycle of Sarcocystis suihominis, feces of naturally infected pigs were collected and over 10000 sporocysts were received by flotation technique, which were mixed with fodder to infect a normal pig. Fresh pork meat containing mature sarcocysts was chopped into pieces and swallowed by a volunteer （the first author of this paper） with about 71 000 sporocysts. Symptoms and development of the parasites after infection were observed. Results The volunteer showed abdominal distension in about 5 hours after infection, with watery diarrhea 13 times from the 8th to 36th hour, vomiting 4 times, chilling and fever with a temperature of 38.5%, dizziness, headache, joint and muscle ache, epigastralgia, and anorexia. Un-sporized sporocysts were found in the faces 10 days after infection and sporocysts appeared on the 12th day. The average size of sporocysts was 11.9（8.8-14.5）μm×9.2 （7.5-12.5） μm. The infected pig showed a slight anorexia, fatigue, constipation, hair loosen in 5-8 days after infection, and returned normal on the 17th day. The average size of the sarcocysts was 299.2（175-575）μm×62.3（30- 102.5）μm. Size of bradyzoites was 11.5（9.5-13.5）μm×4.1（2.8-5.0）μm. The volunteer was treated with acetylspiramycin for 15 days （0.2 g/time, 4 times/d） after 46 days of infection, and fecal examination turned negative 30 days later. Conclusion There is a man-pig cycle for Sarcocystis suihominis in Guangxi.

关 键 词：猪人肉孢子虫 感染 广西 

分 类 号：R382.3[医药卫生—医学寄生虫学]