基因表达系列分析及其在寄生虫学研究中的应用  

Serial Analysis of Gene Expression in Parasitological Research

作　　者：李巧丽[1] 张志明[1] 乔中东[1] 

机构地区：[1]上海交通大学生命科学技术学院,上海200240

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第6期504-509,共6页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：基因表达系列分析(serialanalysisofgeneexpression,SAGE)是一种高通量、快速定量分析真核细胞基因表达信息的技术。它通过抽取距离每个mRNA中3′端polyA尾最近的一个锚定酶识别位点下游的10～14bp片段作为其代表性标签,连成长串,克隆到载体中,进行高效测序,统计出研究对象中的mRNA的种类和丰度。SAGE技术不仅能够全面地分析特定组织或细胞表达的基因并得到这些基因表达丰度的数量信息,还可比较不同组织、不同时空条件下基因表达的差异。本文将综述基因表达系列分析技术的原理、特点、方法、进展及其在寄生虫学研究中的应用。Serial analysis of gene expression （SAGE） is a powerful high-throughput experimental technique that allows rapid, quantitative analysis of global gene expression in eukaryotie organisms. A short sequence taq （10-14bp）, which is defined by an anchoring enzyme site at a fixed distance from polyA tail, contains sufficient information to identify mRNA transcript from which it originates. The taqs are ligated to obtain eoneatemers that are cloned into a plasmid vector for sequencing. The identification and abundance of mRNA can be observed through bioinformaties and statistical analysis of a given tag. SAGE is not only applied in obtaining global profile of gene expression in a given cell or tissue, but also help identify a set of specific genes to the cellular conditions by comparing the profiles constructed for a pair of cells that are kept at different conditions. This review covers a general introduction of SAGE, its protocol, methodological evolution and applications in parasite biology.

关 键 词：基因表达系列分析 mRNA表达谱 寄生虫学 

分 类 号：R38[医药卫生—医学寄生虫学]