从噬菌体肽库中筛选弓形虫抗原模拟表位初探  

Screening of Mimic Epitopes of Toxoplasma gondii Antigen by Phage-displayed Peptide Library

作　　者：何艳燕[1] 张述义[1] 朱民[1] 钱旻[2] 谭红岩[2] 

机构地区：[1]上海市疾病预防控制中心,上海200336 [2]华东师范大学生命科学院,上海200062

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第6期515-517,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：上海市卫生局基金(No.01419)~~

摘　　要：以弓形虫感染兔血清免疫球蛋白(Ig)作为靶分子,免疫筛选噬菌体随机7肽库和12肽库。经3轮筛选,随机挑取70个噬菌体克隆用ELISA检测其特异性,共获得43个阳性克隆。对23个ELISA反应较强的克隆进行测序,根据序列分析选择有代表性的克隆A7作为模拟表位抗原进行ELISA检测。对47份弓形虫感染兔血清进行ELISA检测,结果32份为阳性,敏感性为68.1%;155份健康人血清中弓形虫抗体呈阳性者10份,特异性为93.5%。从噬菌体随机肽库中能筛选到弓形虫抗原模拟表位有诊断弓形虫病的潜在价值。Specific antibodies purified from sera of rabbit infected with Toxoplasma gondii were used to immunoscreen the 12-mer and 7-mer phage random peptide libraries. By 3 rounds of screening, 70 clones were picked out randomly to test the specificity and 43 were found positive by ELISA. 23 of the 43 positive clones showing strong reaction in ELISA were picked out for sequencing. Clone A7 was selected as mimic antigen in ELISA test after analysis of the 23 short peptide sequences. 23 out of 47 sera from rabbits infected with Toxoplasma gondii were ELISA positive by A7 mimic antigen with a positive rate of 68.1%, and 10 of 155 sera from healthy persons showed false positive with a specificity of 93.5%. The primary result suggests that mimic antigen may have a potential use in diagnose of toxoplasmosis.

关 键 词：弓形虫 噬菌体 肽库 

分 类 号：R382.5[医药卫生—医学寄生虫学]