溶石颗粒剂对结石模型大鼠肾骨桥蛋白mRNA表达的影响  

Effect of Rongshi Granules on Renal Stone Formation and Osteopontin mRNA Expression in a Rat Urolithiasis Model

作　　者：黄萍[1] 黄卫华[1] 孔繁智[1] 楼宜嘉[2] 

机构地区：[1]浙江省立同德医院,杭州310012 [2]浙江大学药学院,杭州310031

出　　处：《中国现代应用药学》2007年第6期448-452,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省分析测试基金(04032)

摘　　要：目的通过测定用药前后肾脏骨桥蛋白(osteopontin,OPN)mRNA含量的变化,阐明民间验方溶石颗粒剂治疗肾结石的作用机制。方法采用乙二醇、氯化胺法致大鼠肾结石,同时每天一次ig给予溶石颗粒剂5.8,11.6,17.4g.kg-1,实验全程21d。每7d做以下测定:血标本测定尿素氮(BUN)、肌酐(Cr)、钙和磷含量;尿标本以EDTA法测定钙含量,比色法测定草酸含量;肾组织HE染色,逆转录聚合酶链式反应(RT-PCR)技术检测大鼠肾骨桥蛋白mRNA的表达。结果模型组大鼠肾OPN mRNA的表达明显增加,与溶剂对照组相比(P<0.01),其中21d模型组OPN mRNA的表达最高,为溶剂对照组的4倍;溶石颗粒剂各剂量组均能明显抑制大鼠肾结石模型OPN mRNA的表达(P<0.05),且呈现明显的量效关系;溶石颗粒剂各剂量组能减轻大鼠肾脏草酸钙结晶程度,显著降低大鼠尿钙和草酸含量(P<0.01)。结论溶石颗粒剂可以抑制大鼠肾结石模型中OPN mRNA的表达。OBJECTIVE To investigate the effect of Rongshi Granules on osteopontin （OPN） mRNA expression. METHODS The stone group rats were administered with ethylene glycol （EG） and ammonium chloride, The control group rats were non-treated; and the Rongshi Granule groups （low-dose, medium-dose and high-dose, groups） were administered with Rongshi Granule in addition to EG and ammonium chloride in 21 days. Pooled 24-hour urine samples from each group were collected weekly by the use of metabolic cages, the concentration of uric calcium and oxalic acid were respectively measured by EDTA and photoelectric colorimetric method. Eight animals from each group were killed at 0, 7, 14, and 21 days, kidney was used for histologic examination and the expression of osteopontin mRNA was analyzed with RT-PCR technology. RESULTS The expression of kidney osteopontin mRNA in model group was obviously higher than that of in solvent control group （P 〈 0.01 ） , and was the highest at 21 days, and 4 times as that of in control. The expression of kidney osteopontin mRNA in all of the Rongshi Granule groups was lower than that of in model group （ P 〈 0.05）, with an obvious dose-dependent manner. The eaystalization degree of the kidney calcium oxalate of the rats in all the Rongshi Granule groups was much lower than that of model group, and the uric calcium and oxalic acid of all the Rongshi Granule groups were much lower than that of model group （P 〈 0.01 ）. CONCLUSION The Rongshi Granules could inhibit the expression of osteopontin mRNA in a rat urolithiasis model.

关 键 词：溶石颗粒剂 肾结石 骨桥蛋白mRNA 草酸钙 

分 类 号：R965.1[医药卫生—药理学] R289[医药卫生—药学]