穿心莲生长过程中内酯类成分的积累动态及其相关性研究  

Studies on the Dynamic Accumulations and Correlations of Lactone in the Growth of Andrographis paniculata(Burm.f.) Nees

作　　者：曾令杰[1] 梁晖[1] 林蔚兰[2] 

机构地区：[1]广东药学院中药学院 [2]广州白云山和记黄埔中药有限公司,广州510515

出　　处：《中国现代应用药学》2007年第6期464-467,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立穿心莲药材中内酯类成分的HPLC测定方法,对穿心莲生长过程中内酯成分的积累动态进行研究,探讨内酯成分的积累与其他性状之间的相关性。方法以YileteODS C18柱(5μm,4.6mm×250mm);甲醇-水(18:82)为流动相,梯度洗脱,226nm为检测波长,进行穿心莲内酯类成分的HPLC分析,以spss13.0统计软件进行相关性评价。结果按照上述方法,对其中的穿心莲内酯和脱水穿心莲内酯进行了定量分析,以SPSS13.0统计软件分析了穿心莲内酯类成分的含量之间及其与其他性状之间的相关性。通过分析,发现不同生长时期的穿心莲内酯含量变化较大,在初期增长最快,初花期(生长150d左右)达到最高,脱水穿心莲内酯含量的变化规律不明显,积累缓慢;相关性分析表明,穿心莲内酯的含量与叶片的数量、植株高度、乙醇浸出物、叶片干重和植株干重以及生长发育时期具有显著的相关性,而脱水穿心莲内酯与这些性状之间无明显的相关性。结论穿心莲内酯类成分的HPLC分析方法分离度好、准确性高,适宜于穿心莲药材的内酯类成分的含量测定。OBJECTIVE To establish a simple and accurate HPLC method for the study of lactone accumulation of A. paniculata and to study the correlations of the lactones with other characters. METHODS Detected at 226nm, analytes were separated on a Yilete ODS C18 column （ 150 mm ×4.6 mm,5 μm） eluted gradually with methanol and water （ 18： 82）, with methanol gradually increased from 18% to 60% in the whole analysis. After determination, SPSS 13.0 software was used to calculate the correlations of lactones with other characters. RESULTS Under the above conditions, the lactone HPLC method of Andrographis paniculata was constructed, and through the software SPSS 13.0, the correlations of andrographolide and 14-deoxy-11, 12-dehydroandrographolide with other characters were calculated. Through determination and analysis, it was found that during the growth period the contents of andrographolide varied greatly, it accumulated rapidly in the early growth period, and reached highest level at initial flower development period （about 150th day of its growth） ; while the accumulation of 14-deoxy- 11,12-dehydroandrographolide was very slow. The analysis of correlation coefficients suggested that the contents of andrographolide were significantly correlated with leaf number, dry weight, plant height, dry weight of leaf istics, alcohol extract and growth time; while the content of 14-deoxy-11,12-dehydroandrographohde had almost no correlation with these characters. CONCLUSION The developed method for analysis of the lactones, accurate and reproducible, was suitable for the quality evaluation of Herba Andrographitis.

关 键 词：穿心莲 穿心莲内酯 脱水穿心莲内酯 相关性 

分 类 号：R93[医药卫生—生药学] R917.101[医药卫生—药学]