产舒康颗粒中盐酸水苏碱的高效液相色谱法定量  

Determination of Stachydrine Hydrochloride in Chanshukang Granules by HPLC

作　　者：程立方[1] 崔秀君[2] 

机构地区：[1]山东省中医药研究院,济南250014 [2]济南市妇幼保健院,济南250001

出　　处：《中国现代应用药学》2007年第6期496-498,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：济南市卫生局科技计划项目(WS20011215)

摘　　要：目的建立产舒康颗粒中盐酸水苏碱的高效液相色谱法定量方法。方法通过强酸性阳离子交换树脂柱得到产舒康颗粒中盐酸水苏碱。高效液相色谱法测定条件:Spherisorb SCX柱150mm×4.6mm,5μm;流动相:甲醇-水(60:40)含0.05mol.L-1磷酸二氢钾;检测波长:215nm。结果盐酸水苏碱在1.0～5.0μg内线性关系良好,平均回收率96.21%,RSD=2.22%,三批颗粒中盐酸水苏碱含量分别为0.0304%,0.0276%,0.0191%。结论该方法简便、灵敏、准确,为控制产舒康颗粒的质量提供依据。OBJECTIVE To establish a method for determination of stachydrine hydrochloride in Chanshukang granules. METHODS The substance was extracted through a strong acidic cation resinedumn. Determination conditions of HPLC was as follows. Stationary phase： Spherisorb SCX column 150 mm ×4.6 mm,5μm; mobil phase： methanol-water（60： 40） containing 0.05 mol · L^-1 KH2PO4 ;detection wavelength ：215 nm. RESULTS There is a good linear relationship within the range of 1.0 ～ 5.0 μg for staehyarine hydrochloride. The average recovery was 96.21% , RSD = 2.22%, The contents in three batches were 0. 0304% ,0.0276%, 0. 0191% ,respectively. CONCLUSION The method is simple, sensitive,accurate and provides evidence for controlling the quality of Chanshukang Granules .

关 键 词：产舒康颗粒 盐酸水苏碱 高效液相色谱法 

分 类 号：R917.78[医药卫生—药物分析学]