牛蛙蝌蚪变态发育中的蛋白质差异表达  

The Differential Expression of Rana Catesbeiana tadpole's Proteins During Metamorphosis

作　　者：范丽华[1] 虞鹏程[1] 王三英[2] 

机构地区：[1]南昌大学生命科学学院,江西南昌330031 [2]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《南昌大学学报（理科版）》2007年第5期486-489,共4页Journal of Nanchang University(Natural Science)

摘　　要：采用双向电泳和质谱技术对牛蛙蝌蚪变态发育中(I、II、III期)肌肉总蛋白质进行了研究。获得了10个与牛蛙蝌蚪变态相关的差异性蛋白。对10个差异蛋白进行质谱鉴定及数据库的检索后,发现了参与细胞分化及胚胎发育的锌指蛋白XLCOF22、参与蛋白质合成的转录启动子RAP30和初期发育蛋白EGR2-XENLA。Two -dimensional electrophoresis and mass spectrometry tenichques were developed to analyse comparatively the expression of muscle proteins of Rana Catesbeiana tadpole in three different metamorphosis stages （ tadpole, prometamorphosis, climax of metamorphosis）. The results indicated that there were differential expressions at vary stage and 10 spots were obtained. The differential exprssion proteints were primarily identified by mass spectrometry and peptident software searched on the SWISS - PROT database. Three proteins which are likely to be relevant to the key genes that regulate the metamorphosis of Rana Catesbeiana tadpole were identified,zinc finger protein XLCOF22 ,transcription initiaion factor RAP30 and early growth response protein2 EGR -2.

关 键 词：牛蛙 蝌蚪 蛋白质组学 双向电泳 质谱 

分 类 号：Q51[生物学—生物化学]