高质量提取大豆叶片总RNA的改良方法  

Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves

作　　者：姚滢秋[1] 陈星[2] 王璞[1] 梁燕[2] 闫梅[2] 肖白[2] 

机构地区：[1]首都师范大学生命科学学院,北京100037 [2]首都医科大学附属北京朝阳医院基础医学研究中心,北京100020

出　　处：《生物技术通报》2008年第1期133-135,共3页Biotechnology Bulletin

摘　　要：豆科植物总RNA的提取相对比较困难,大豆叶片富含多糖、蛋白质,传统方法提取效果均不理想。在总RNA的提取方法Trizol法和热酚-氯化锂沉淀法的基础上,针对大豆叶片的特殊性,做了适当的改良,从而获得了完整性好,高纯度的大豆叶片总RNA,并成功进行了基因片段的RT-PCR实验,结果证实所获得的RNA完全可以适应后续实验要求。Tissue of Glycine max leaves contain polysaccharide and other secondary metabolites in great quantity that making RNA difficult to extract. We present improved protocols based on hot phenol-LiC1 deposition and Trizol method for quick isolation of total RNA with high purity and high integrality. The result shows that the extracted total RNA can meet the demand of the RT-PCR experiment. There fore,these methods deserve further studies.

关 键 词：大豆 RNA 提取方法 

分 类 号：Q943.2[生物学—植物学] Q945.11