微波辅助萃取-高效液相色谱法测定鱼腥草药材中槲皮素的含量  

Determination of content of quercetin in Houttuynfa cordata Thunb by microwave assisted extraction-high performance liquid chromatography

作　　者：陈斌[1] 郁颖佳[1] 归靓[1] 周涛[1] 段更利[1] 

机构地区：[1]复旦大学药学院药物分析教研室,上海200032

出　　处：《复旦学报（医学版）》2008年第1期142-144,共3页Fudan University Journal of Medical Sciences

基　　金：上海市科学技术委员会科研计划项目资助(05dz19741)

摘　　要：目的建立微波辅助萃取-高效液相色谱法（MAE—HPLC）分析鱼腥草药材中槲皮素含量的方法。方法采用Diamonsil C18柱（5μm，150mm×4．6mm），以甲醇-0．2％三氟乙酸（50：50）为流动相，350rim处波长检测，流速1．0mL／min。微波辅助萃取条件的溶剂是10mL甲醇，提取功率为400w，提取时间为4min。结果槲皮素在0．01～0．2μg的范围内线性关系关系良好（r=0．9991），高、中、低3个浓度的平均加样回收率为98．32％～103．22％，RSD为1．21％～2．92％（n=3）；样品的天内和天间RSD为0．42％和2．99％。结论该方法简便、准确，适用于分析测定鱼腥草中槲皮素的含量。Objective To develop an MAE- HPLC method for the determination of quercetin in Houttuynfa cordata Thunb. Methods Quercetin was determined by HPLC on Diamonsil C18 column （150 mm × 4. 6 mm,5 μm） at 30 ℃ with methanol-0.2% trifluoroacetic acid （50：50）as the mobile phase. Detection wavelength was 350 nm. The flow rate was 1.0 mL/min. Quercetin was extracted by MAE with 10 mL methanol using extraction condition of 400 w and 4 min. Results The standard curves for quercetin were linear over the range of 0.01 - 0.2 tLg （r = 0. 999 1）. The average recoveries of quercetin were between 98.32% and 103.22% with RSD from 1.21% and 2.92%（n = 3）. The RSD of intra-day and inter-day were 0.42 % and 2.99 %, respectively. Conclusions The method was simple and accurate. It is adapted to determine and analyze quercetin in Houttuynfa cordata Thunb.

关 键 词：微波辅助萃取 高效液相色谱法 鱼腥草 槲皮素 含量测定 

分 类 号：R286.0[医药卫生—中药学] R284.2[医药卫生—中医学]