岷江百合根尖染色体的C-分带和FISH分析  

Giemsa C-banding and FISH Analysis of Minjiang Lily Chromosome by Means of Root Tips

作　　者：刘光欣[1] 胡凤荣[1] 席梦利[1] 吴祝华[1] 池坚[1] 施季森[1] 

机构地区：[1]国家教育部和江苏省共建林木遗传和生物技术重点实验室,南京林业大学,南京210037

出　　处：《分子植物育种》2008年第1期95-99,共5页Molecular Plant Breeding

基　　金：上海市重点攻关项目(沪农科攻字2006,第4-1号);江苏省自然科学基金项目(164010028);国家林业局“948”项目(2007-4-06)资助

摘　　要：利用GiemsaC-分带和荧光原位杂交(FISH)的方法对岷江百合(L.regale)根尖染色体进行了研究。结果表明岷江百合的带型公式为:2n=24=4I+8CI++2I++6I++2I+T++2I+T+,每条染色体都显示出了可以明显区别的特征带,带纹强弱差异明显。以45SrDNA为探针对岷江百合根尖染色体进行了荧光原位杂交,杂交点数为5对,分别位于A、B、H和K4对同源染色体的着丝点区域和一对G染色体的长臂上。通过GiemsaC-带和FISH的方法可以将岷江百合的每条染色体区分开来。Giemsa C-banding and FISH analysis of the Minjiang lily chromosomes （Lilium regale） from root tips were carried out in this research. The results showed that the C-banding karyotype would be 2n=24=4I＋8CI＋＋2I＋＋ 6I^＋＋2I^＋T＋＋2I＋T^＋. The specific C-banding points on each chromosome could be distinguished and the differences of C-banding intensity also could be recognized. 45S rDNA was used as probe for FISH analysis （fluorescence in situ hybridization）, and 5 couples of hybridization signals were displayed on the chromosome centrimere of chromosome A, B, H, K and the long arm of G chromosome. In this research we has a conclusion that Minjiang lily chromosomes can be easily distinguished and identified by Giemsa C-banding and FISH approaches.

关 键 词：岷江百合 染色体 C-带分析 荧光原位杂交 

分 类 号：S682.29[农业科学—观赏园艺]