放线菌SPRI10304次级代谢产物中杀菌活性物质的分离提取及结构鉴定  

Isolation,Purification and Identification of Fungicidal Active Compound from the Secondary Metabolites of Actinomycete SPRI10304

作　　者：孙军利[1] 王金来[1] 王丽云[1] 陶黎明[1] 

机构地区：[1]华东理工大学药学院,上海200237

出　　处：《化学与生物工程》2008年第1期43-45,51,共4页Chemistry & Bioengineering

基　　金：国家重点基础研究发展计划资助项目(2003CB114402);国家科技支撑计划资助项目(2006BAE01A01-6)

摘　　要：从苏州地区采集的土壤样品中分离得到能产生杀菌活性物质的菌株SPRI10304。采用硅胶柱层析、薄层层析以及制备高效液相色谱等技术对SPRI10304发酵液中的杀菌活性成分进行分离纯化,得到了1个杀菌活性化合物,经IR、UV、LC-MS、NMR等分析,确定该活性物质的结构为2-乙酰基-3-甲氧基呋喃,俗名异麦芽酚甲醚。该活性物质对黄瓜白粉病有较好的防治效果。A Streptornyces strain producing f soil samples collected in Suzhou area. One fungi via silica gel column chromatography, thin layer as 2-acetyl-methoxyfuran by IR,UV, LC-MS and ungicidal active substances,SPRI10304, was isolated from the cidal active compound was separated from fermentation broth chromatography and pre-HPLC. The compound was identified NMR spectra. The result indicated that it had high fungicidal activity against Sphaerotheca fuliginea.

关 键 词：放线菌SPR110304 杀菌活性 呋喃类化合物 

分 类 号：TQ458[化学工程—农药化工]