人脐带间质干细胞的分离纯化及生物学特性鉴定  

Separation,purification and biological characterization of human mesenchymal stem cells from umbilical cord

作　　者：乔纯[1] 许文荣[1] 朱伟[1] 胡嘉波[1] 钱晖[1] 阴晴[2] 杨欢[1] 王兴忠[1] 严永敏[1] 毛飞[1] 陈永昌[3] 

机构地区：[1]江苏大学医学技术学院,江苏镇江212013 [2]江苏大学附属医院临床检验中心,江苏镇江212001 [3]江苏大学医学院,江苏镇江212013

出　　处：《临床检验杂志》2008年第1期5-6,共2页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然基金项目(30471938);江苏省医学领军人才项目;江苏省自然基金重点项目(BK2007705);江苏省自然基金项目(BK2007092);镇江市社会发展科学技术基金(SH2006066;SH2006070)

摘　　要：Objective To isolate mesenchymal stem cells(MSCs)from human umbilical cord and investigate the basic biological characteristics.Methods Human umbilical cord mesenchymal stem cells(hUCMSCs)were separated from culture of tissue adherence and the biological characteristics,including morphologic appearance,surface antigens,growth curve,cell cycle,cytogenetic features,differentiation potential and gene expression,were investigated.Results After two weeks of incubation,fibroblast-like cells appeared to be dominant.During the second passage the cells presented a homogeneous population of spindle fibroblast-like cells.Flow cytometry analysis revealed that CD29,CD44,CD95,CD105 and HLA-I were expressed on the surface of cells,but there was no expression of hematopoietic lineage markers,e.g.,CD34,CD38,CD71 and HLA-DR.Chromosomal analysis showed the cells kept normal karyotype.The cell cycle at the third passage showed the percentage of G0/G1,G2/M and S phase were 88.86%,5.69% and 5.45% respectively.The assays in vitro demonstrated the cells exhibited multipotential differentiation into osteogenic and adipogenic cells.Both BMI-1 and nucleostemin genes,expressed in adult MSCs from bone marrow,were also expressed in hUCMSCs.Conclusion Here we show the umbilical cords may be a novel alternative source of human MSCs for experimental and clinical applications,which almost have the same biological characteristics of human adult MSCs.Objective To isolate mesenchymal stem cells （MSCs） from human umbilical cord and investigate the basic biological characteristics, Methods Human umbilical cord mesenchymal stem cells （hUCMSCs） were separated from culture of tissue adherence and the biological characteristics, including morphologic appearance, surface antigens, growth curve, cell cycle, cytogenetic features, differentiation potential and gene expression, were investigated. Results After two weeks of incubation, fibroblast-like cells appeared to be dominant. During the second passage the cells presented a homogeneous population of spindle fibroblast-like cells. Flow cytometry analysis revealed that CD29, CD44, CD95, CD105 and HLA-I were expressed on the surface of cells, but there was no expression of hematopoietic lineage markers, e.g. , CD34, CD38, CD71 and HLA-DR. Chromosomal analysis showed the cells kept normal karyotype. The cell cycle at the third passage showed the percentage of G0/G1, G2/M and S phase were 88.86%, 5.69% and 5.45% respectively. The assays in vitro demonstrated the cells exhibited muhipotential differentiation into osteogenic and adipogenic cells. Both BMI-1 and nucleostemin genes, expressed in adult MSCs from bone marrow, were also expressed in hUCMSCs. Conclusion Here we show the umbilical cords may be a novel alternative source of human MSCs for experimental and clinical applications, which almost have the same biological characteristics of human adult MSCs.

关 键 词：间质干细胞 脐带 分化 

分 类 号：R446.11[医药卫生—诊断学]