甲氨蝶呤与DNA相互作用的荧光光谱研究  

Study on the Interaction of Methotrexate and DNA by Fluorescence Spectroscopy

作　　者：张爱梅[1] 王怀生[1] 罗玉会[1] 

机构地区：[1]聊城大学化学化工学院,山东聊城252059

出　　处：《分析测试学报》2008年第1期45-48,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20475024);聊城大学科研基金资助项目(X061003)

摘　　要：在pH7.4的Tris-HCl介质中,以盐酸小檗碱为荧光探针,研究了甲氨蝶呤与小牛胸腺DNA之间的相互作用。采用荧光光谱、吸收光谱、Scatchard方程、盐效应等手段,对作用机理进行了研究,结果表明甲氨蝶呤与小牛胸腺DNA之间的作用方式为混合方式,嵌入与静电作用是两种主要作用方式。求得25℃和40℃时二者的结合常数分别为5.61×104和5.32×104L.mol-1。The interaction of methotrexate （MTX） and ct-DNA under simulated human body condition have been studied by fluorescence spectroscopy using berberine（BR） as a fluorescence probe in Tris - HCl buffer solution（ pH 7.4）. The mechanism of the interaction between MTX and ct-DNA was also investigated by fluorescence spectrum, absorption spectrum, Scatchard equation, and salt effect. Experimental results showed that the interaction mode was of a mixed one and that the intercalation and electrostatic binding were the two major modes. The determined average binding constants were 5.61×10^4L·mol^-1 at 25 ℃ and 5.32×10^4L·mol^-1 at 40 ℃.

关 键 词：荧光光谱法 脱氧核糖核酸(DNA) 甲氨蝶呤 小檗碱 

分 类 号：O657.32[理学—分析化学] Q523[理学—化学]