荧光素的共振光散射光谱研究  

Study on the Resonance Light Scattering of Fluorescein

作　　者：曾文[1] 李松青[1] 韩一秀[1] 秦惠[1] 

机构地区：[1]湘潭大学化学学院,湖南湘潭411105

出　　处：《分析测试学报》2008年第1期70-72,75,共4页Journal of Instrumental Analysis

摘　　要：研究了荧光素(Flu)的共振光散射(RLS)光谱的形成机理与影响因素。在中性和碱性条件下,Flu溶液的共振散射光显著增强。实验发现,随着溶液pH的增加,Flu溶液的RLS光谱与其荧光光谱、吸收光谱在强度大小、最大吸收峰位移上变化趋势一致。荧光素的荧光激发光谱与发射光谱有部分重叠,共振散射峰处于荧光激发峰与荧光发射峰之间。在光偏振实验中,测得共振散射光的偏振度P≈0.020。碱性环境中,随Flu溶液浓度的增加,其RLS光谱和荧光光谱的变化情况,同样表明两者之间有密切联系。上述实验结果揭示Flu的共振散射光就是共振荧光。The generation mechanism of resonance light scattering（RLS） spectra of fluorescein（Flu） and the influencing factors were studied in detail. In neutral and alkaline solutions, the RLS intensity of Flu was enhanced significantly. Experimental results showed that the extent of the variations of the intensities and wavelength shifts of RLS, fluorescence and absorption spectra with the increase of pH were the same. The fluorescence excitation and emission spectra of Flu overlap partially, and the RLS peak is in the midst of fluorescence excitation and emission peaks. It was found that the polarization degree was measured to be P ≈0. 020. The variations of RLS spectra and fluorescence spectra with the increase of Flu concentration in strong alkaline solutions were similar. Based on the above phenomena, the RLS of Flu was confirmed to be the resonance fluorescence.

关 键 词：荧光素 共振光散射 荧光光谱 吸收光谱 光偏振 

分 类 号：O657.32[理学—分析化学] TQ617.3[理学—化学]