羟丙哌嗪分子印迹的固相萃取  

Solid Phase Extraction of Dropropizine Using Molecularly Imprinted Polymers

作　　者：耿利娜[1] 康宁[1] 宁周云[1] 武利庆 李琰[1] 孙立权[1] 罗爱芹[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100029 [2]中国计量科学研究院,北京100013

出　　处：《物理化学学报》2008年第1期25-31,共7页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金(20705005);北京理工大学基础研究基金(1060050320708)资助项目

摘　　要：以L-羟丙哌嗪为模板分子,!-甲基丙烯酸为功能单体,三羟甲基丙烷三甲基丙烯酸酯为交联剂,合成了羟丙哌嗪分子印迹聚合物,利用该聚合物进行羟丙哌嗪固相萃取应用研究.实验证明,所合成的分子印迹聚合物具有良好的识别萃取模板分子羟丙哌嗪的能力,能够用于羟丙哌嗪的富集浓缩,而空白聚合物却不具备这样的特性.考察了模板分子与其结构类似物苄基哌嗪、N-异丙基哌嗪和乙氧基羰基哌嗪的固相萃取分离情况,研究表明,虽然其中的苄基哌嗪在印迹材料上具有最强的保留,但是通过先后使用不同的洗脱溶液实现了模板分子与苄基哌嗪的选择性分离.探讨了印迹识别机理,并用PM3半经验计算机模拟方法进行了辅助分析验证.Molecularly imprinted polymer （MIP） against dropropizine was synthesized using dropropizine as template, α-methyl acrylic acid as functional monomer, and trimethylolpropane trimethacrylate as crosslinking agent. Its application for separation and enrichment in solid phase extraction was investigated. It was found that, the imprinted polymer exhibited satisfactory performance in recognition and enrichment towards template, but it was not the case with non-imprinted polymer. The retention and separation of imprinted molecule with its structural analogues were also studied, The selectively separation was proved to be successful. The mechanism of imprinted recognition was also discussed here. With the help of PM3 semi-empirical method, the possible MIP system was simulated and compared with experimental results.

关 键 词：羟丙哌嗪分子印迹 固相萃取分离 结构类似物 PM3计算机模拟方法 

分 类 号：O658.2[理学—分析化学]