降解前后异枝麒麟菜硫酸多糖对草酸钙晶体生长的调控作用  

Modulation of Eucheuma Striatum Sulfated Polysaccharide before and after Degradation on Crystal Growth of Calcium Oxalates

作　　者：王淼[1] 于海燕[1] 欧阳健明[1] 

机构地区：[1]暨南大学生物矿化与结石病防治研究所,广州510632

出　　处：《物理化学学报》2008年第1期109-114,共6页Acta Physico-Chimica Sinica

基　　金：广东省科技攻关项目(2006B36501001);国家自然科学基金(30672103;20471024);教育部留学基金(教外司[2005]55号)资助

摘　　要：采用过氧化氢法降解产于印尼的海藻异枝麒麟菜硫酸多糖(ESPS).降解前ESPS的平均分子量为1410000,硫酸基(OSO23-)含量为16.0%(w);而降解后其分子量显著下降至4819,硫酸基含量则略微下降至15.2%.采用体外模拟方法研究了降解前后ESPS对草酸钙晶体生长的抑制作用.扫描电子显微镜(SEM)和X射线衍射(XRD)结果表明,降解后ESPS能使一水草酸钙(COM)晶体数量进一步减少,晶粒尺寸进一步减小.当降解ESPS浓度从0.006g·L-1分别增加到0.010和0.050g·L-1时,COM晶体平均尺寸由11.5"m×2.9"m分别减小到9.2"m×2.9"m和6.0"m×2.5"m,晶体数量由3357mm-2分别减少到2298和1532mm-2,COM的(101)面衍射峰强度I(101)与(010)面对应的(020)衍射峰强度I(020)之比(I(101)/I(020))由5.54分别增加到16.2和20.0.结果显示,具有小分子量的降解ESPS对草酸钙晶体成核与生长的抑制效果明显优于大分子量的未降解ESPS.Algal eucheuma striatum sulfated polysaccharide （ESPS） collected from Indonesian sea was degraded by hydrogen peroxide （H2O2）. After degradation the average molecular weight of ESPS was decreased from 1410000 to 4819, and the content of sulfate （OSO3^2-） was lightly decreased from 16.0% to 15.2% （w）. The effect of ESPS before and after degradation on the growth of calcium oxalate crystal was investigated in vitro by using scanning electron microscopy （SEM） and X-ray diffraction （XRD） analysis. Compared with the undegraded ESPS, the size and number of calcium oxalate monohydrate （COM） crystal induced by the degraded ESPS were much smaller. With increasing the concentration of degraded ESPS from 0.006 g· L^-1 to 0.010 and 0.050 g· L^-1, the average size of COM crystals decreased from 11.5 μm×2.9 μm to 9.2 μm×2.9 μm and 6.0 μm×2.5 μm, respectively, and the number of COM crystals decreased from 3357 per mm^2 to 2298 and 1532 per mm^2, and the diffraction peak intensity ratio of the （1^-01） plane to （010） plane of COM crystals （I1^01/I（020）） increased from 5.54 to 16.2 and 20.0. The result showed that the inhibition efficiency of the degraded ESPS with a low-molecular-weight on the nucleation and growth of CaOxa crystal was obviously superior to that of the undegraded ESPS.

关 键 词：海藻异枝麒麟菜 小分子量多糖 降解 尿结石 草酸钙 

分 类 号：O781[理学—晶体学]