二脱氧肌苷引发培养的背根神经节神经元的形态学改变(英文)  

Morphological alterations induced by didanosine in cultured dorsal root ganglion nrurons

作　　者：刘花香[1] 刘真[2] 杨向东[3] 王丽红[2] 李振中[2] 

机构地区：[1]山东大学齐鲁医院风湿内科,济南250012 [2]山东大学医学院解剖学教研室,济南250012 [3]山东大学齐鲁医院肾内科,济南250012

出　　处：《神经解剖学杂志》2008年第1期8-12,共5页Chinese Journal of Neuroanatomy

基　　金：教育部留学回国人员科研启动基金(外教司留[2003]406号);山东省优秀中青年科学家奖励基金(02BS091)资助项目;山东省自然科学基金(Z2006C06,Z2006D05)重点资助项目

摘　　要：为探讨二脱氧肌苷(ddI)对培养的背根神经节(DRG)神经元的影响,我们对分散培养的胎鼠DRG神经元培养3d后,再分别以不同浓度的ddI(1μg/ml,5μg/ml,10μg/mll和20μg/ml)孵育3d。终止培养后,行微管相关蛋白2(MAP2)标记,用共聚焦激光扫描显微镜观察神经元胞体和突起的改变。结果表明,DRG神经元用ddI孵育3d,神经元突起的数目减少和长度变短,呈剂量依赖性,而神经元的直径则没有变化。本研究的结果表明,ddI可影响培养的DRG神经元突起的再生和生长。To investigate the effects of didanosine （ ddl ） on the morphological alterations of dorsal root ganglion （DRG） neurons, dissociated DRG cells from rat embryo were studied. DRG cells were cultured for 3 days and then treated with ddl for additional 3 days in different concentrations （ 1 μg/ml, 5 μg/ml, 10 μg/ml and 20μg/ml, respectively）. After that, DRG cells were processed for microtubule associated protein 2 （ MAP2 ） labeling and observed under confecal laser scanning microscopy （ CLSM ）. The results showed that both the number and length of neurites of the DRG cells after exposed to ddl significantly down-regnlated in a dose-dependent manner compared with control group, thus suggesting that ddl may have inhibitory effects on neurite regeneratian and outgrowth in dissociated DRG cultures.

关 键 词：二脱氧肌苷 背根神经节 神经元 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]