Klotho基因G-395A多态性与动脉硬化的相关性研究  

Association of G-395A polymorphism of human Klotho gene with arteriosclerosis

作　　者：王虎林[1] 司良毅[1] 孙兰英 孟萍[1] 肖航[1] 

机构地区：[1]第三军医大学西南医院老年病科,重庆400038 [2]解放军第四十四医院内科

出　　处：《中华老年心脑血管病杂志》2008年第2期96-98,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的研究重庆市汉族人群Klotho基因启动子区域G-395A单核苷酸多态性的分布，探讨该多态性住点与动脉硬化的相关性。方法232倒健康体检者均进行臂踝脉搏波传导速度测定，并记录动脉硬化的传统危险因素，根据测定结果分为动脉硬化组（130例）和对照组（102例）。应用TaqMan探针等位基因特异性杂交分析法对Klotho基因G-395A多态性位点进行分析。结果G-395A多态性住点共检测出GG、GA、AA3种基因型，频率分别为60．3％、34．1％和5．6％，符合Hardy-Weinberg平衡。动脉硬化组-395A等位基因的频率显著低于对照组（33．1％ VS 48.0％，P=0．022）。logistic回归分析，调整传统危险因素后-395A与动脉硬化呈负相关（P=0．042，OR=0．537，95％CI：0．295-0．977）。结论Klotho基因-395A等位基因可能是动脉硬化的遗传学保护因素。Objective To investigate the distribution of G-395A polymorphism in promoter of Klotho gene in Chongqing Han population in China and analyze its association with arteriosclerosis. Methods Genotyping of G-395A polymorphism was performed in 130 arteriosclerotic patients and 102 controls using an allelic discrimination assay with TaqMan probes. Brachial-ankle pulse wave velocity was measured as a marker of atherosclerotic vascular damage. Results Genotype frequencies of GG,GA and AA were 60.3%,34.1% and 5.6% respectively. The distribution was in accordance with Hardy-Weinberg equilibrium. The frequency of the A allele carriers was signif- icantly lower in the arteriosclerosis group than in the control group （33.1% vs 48.0%, P=0. 022）. Logistic regression analysis revealed that after adjusted for traditional risk factors,the A allele was negatively correlated with arteriosclerosis （P=0.042, OR=0.537,95 % CI ,0.295-0.977）. Conclusion The A allele of G-395A polymorphism of Klotho gene may be a protective factor against arteriosclerosis.

关 键 词：动脉硬化 多态性 单核苷酸 启动区(遗传学) 

分 类 号：R543.5[医药卫生—心血管疾病]