双向凝胶电泳分析感染禽脑脊髓炎病毒鸡脑组织蛋白表达图谱  

Analysis of protein expressions of chicken's brain tissue infected with avian encephalomyelitis virus by two-dimensional gel electrophoresis

作　　者：孟佩俊[1] 刘丽华[1] 张丽萍[2] 王嘉慧[1] 陈富荣[1] 何翠[1] 赵娜[1] 

机构地区：[1]内蒙古工业大学化工学院,内蒙古呼和浩特010051 [2]包头医学院公共卫生学院,内蒙古包头014010

出　　处：《中国预防兽医学报》2008年第2期100-103,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：内蒙古工业大学重点项目(ZD2003/0)

摘　　要：本研究采用双向凝胶电泳分离接毒组(感染AEV)和对照组(不接毒)的鸡脑组织全蛋白,经过银染后,用Image ScannerⅡ光密度扫描仪获取凝胶图像,并用Image Master 2D Platinum软件进行差异蛋白分析。对蛋白斑点编辑后,接毒组中检测到793个蛋白点,对照组中检测到827个蛋白点,两张图谱的匹配蛋白点数为614个,凝胶匹配率为75.8%。差异蛋白分析后,发现在接毒组中有16个蛋白点表达量显著下调,这些蛋白质成份可能与组织细胞病变有关,它们将为认识禽脑脊髓炎的发病机理提供线索。The protein expression profile of brain tissues of the AEV-infected chicken were investigated by two-dimensional gel electrophoresis. The 2-D gels were silver stained and the digitized 2-D images were obtained using Image Scanner Ⅱ. The differential proteins expression analysis was carried out using the ImageMaster 2D Platinum software （version 5.0, Amersham）. A total of 793 protein spots were detected for the AEV-infected chickens, while 827 protein spots detected for the uninfected controls. Two gels matching showed that 614 protein spots representing 75.8 % ptoteins were matched. In addition, expression of 16 proteins in the AEV-infected chichens were found significantly down-regulated. These proteins may be related to pathological changes in the brain tissues and would be useful candidates for further pathogenesis study.

关 键 词：蛋白质组 双向凝胶电泳 禽脑脊髓炎 鸡脑组织 

分 类 号：S852.6[农业科学—基础兽医学]