一株鸡马立克氏病毒的分离鉴定及其主要致病基因分析  

Isolation and identification of a field strain of Marek's disease virus

作　　者：张艳萍[1] 刘长军[1] 施维松[1] 秦运安[1] 张晓巍[1] 李晶梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2008年第2期109-112,共4页Chinese Journal of Preventive Veterinary Medicine

摘　　要：吉林省某地区鸡马立克氏病(MD)疫苗免疫鸡群暴发MD,从发病鸡羽髓中分离到一株适应鸡胚成纤维细胞生长的马立克氏病毒(MDV)。该毒株感染无特定病原体(SPF)鸡可引起典型的MD临床症状;对非免疫鸡和火鸡疱疹病毒(HVT)疫苗免疫鸡的致病率分别为76%和72%,二者无显著差异,表明HVT疫苗对该毒株不能提供有效保护。通过对该毒株病毒基因组中132bp重复序列、meq基因的核苷酸及推导的氨基酸序列分析,发现该毒株的132bp重复序列的拷贝数、meq基因的变异符合高毒力MDV毒株的特点。A Marek＇s disease （MD） virus （MDV） strain was isolated in Jilin province from diseased chickens that had been vaccinated by turkey herpesvirus. The isolate was adapted to grow in chick embryo fibroblasts （CEF）. Artificial infection of SPF chickens with the virus produced typical clinical symptoms of MD. The isolate was highly pathogenic and caused 76 % and 72 % morbidity in unvaccinated and HVT-vaccinated chickens, respectively. Analysis of 132 bp repeat sequence and meq gene sequences of the viral genome showed that the virus isolate belongs to the highly virulent MDV strains

关 键 词：马立克氏病毒 分离和鉴定 MEQ基因 132 bp重复序列 

分 类 号：S852.65[农业科学—基础兽医学]