低氧对人骨髓间充质干细胞HIF-1α、SDF-1、VEGF mRNA表达的影响  

Effects of low oxygen on expression of mRNA of HIF-1α,SDF-1 and VEGF in hBMSCs

作　　者：王庆德[1] 杨述华[1] 杨操[1] 胡勇[1] 赵继军[1] 张景辉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,湖北武汉430022

出　　处：《山东医药》2008年第3期21-22,共2页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30170945)

摘　　要：目的观察低氧对人骨髓间充质干细胞(hBMSCs)缺氧诱导因子-1α(HIF-1α)、基质细胞衍生因子-1(SDF-1)及血管内皮细胞生长因子(VEGF)表达的影响。方法分别在1%O2及20%O2条件下培养hBMSCs,定量RT-PCR技术检测其HIF-1α、SDF-1及VEGF mRNA。结果在1%O2条件下,hBMSCs的HIF-1α、SDF-1、VEGF mRNA表达水平明显增高(P<0.01)。HIF-1αmRNA与SDF-1 mRNA及VEGF mRNA的表达密切相关(r分别为0.488、0.644,P均<0.01),但SDF-1 mRNA与VEGF mRNA的表达无明显关系(r=0.077,P>0.05)。结论低氧可促进hBMSCs的HIF-1α表达,并使SDF-1、VEGF mRNA表达增加。Objective To observe the effects of low oxygen on the expression of mRNA of hypoxia-inducible factor- 1α（HIF-1α） ,stromal cell derived factor-1 （ SDF-1 ） and vascular endothelial growth factor（VEGF） in hBMSCs. Method The third passages of hBMSCs were cultured in 1% 02 and 20% O2, HIF-1α,SDF-1 and VEGF mRNA were detected by RT-PCR. Results Cultured under 1% O2 condition, the mRNA of HIF-1α,SDF-1 and VEGF of hMSCs increased greatly （P 〈 0.01 ）. There was obvious association between the expression of HIF-1 α, SDF-1 and VEGF m RNA（ P 〈 0.01 ）, but no correlation were found between SDF-1 and VEGF mRNA expression. Conclusion Low oxygen induces the expression of HIF-1α,and results in increasing SDF-1 and VEGF expression in hBMSCs.

关 键 词：骨髓间充质干细胞 缺氧 缺氧诱导因子 基质衍生因子 血管内皮细胞生长因子 

分 类 号：Q813.11[生物学—生物工程]